FastQCFastQC Report
Fri 14 Jul 2023
SRR9691778_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691778_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10259103
Total Bases779.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAGATCGGAAGAGCACACG3921473.8224296997505536No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC3572873.48263391058653Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGAGATCGGAAGAGCA2508152.444804384944766No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG1213231.182588770187803No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA850160.828688434066799Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA710380.6924387054111846No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT703650.6858786776972606Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAGATCGGAAGAGCACAC628400.6125291850564324No Hit
ATTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGAGATCGGAAGAGC566070.5517733860357966No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT532700.5192461758108872Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA495880.4833560984815144Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC450460.43908322199318983No Hit
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGATCGGAAGAGCACA443440.4322405184936734No Hit
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAGATCGGAAGAGCACAC416790.4062635885418052No Hit
ATTTTCTGGCAAGCCTGGCATTTCGTGAGATCGGAAGAGCACACGTCTGA403290.39310454335042744Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC401850.3917009118633471Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAGATCGGAAGAGCACA399170.38908859770683657No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG322470.31432572613804544No Hit
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC299350.29178964281770053Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCATCATTCTTCTGGTGTAGATCTGAGATCGGAAGAGCACACGTCTGAA297140.2896354583826676Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT293480.2860678950196718Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG292430.28504441372700906No Hit
TCCGATGCTGGCAGATCGTACCTTTGCACAGATCGGAAGAGCACACGTCT262870.25623097847833287Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA259560.25300457554622463No Hit
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGAACGAGATCGGAAG235870.22991288809557717No Hit
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGAACAGATCGGAAGA225370.21967807516894994No Hit
AGCCGTATCAGGACCAGACCAGATCGGAAGAGCACACGTCTGAACTCCAG210200.20489120735019428Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCCA200980.1959040668565273Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCC194750.1898314111867285Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAAC193670.18877868757141827Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC182760.17814422956860848Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC180380.175824338638573No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA176870.17240298688881472No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA176870.17240298688881472No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC175250.17082390146584941No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC170270.16596967590636336No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA163530.15939990075155694No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC161320.15724571631652398No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA159220.1551987537311985TruSeq Adapter, Index 10 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAAGATCTCGTATG155400.15147523131408272TruSeq Adapter, Index 7 (97% over 35bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT137060.13359842473557387No Hit
ATTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGATCGGAAGAGCAC131280.12796440390548763No Hit
AGCCGTATCAGGACCAGACCTATCAGATCGGAAGAGCACACGTCTGAACT129870.12659001474105486Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCTGAAAGGTCTGTATGCAACCCATGCAAGATCGGAAGAGCACACGTC124650.12150185060038875No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA122910.11980579588683339Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA121770.11869458762622814Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG120400.11735918822532536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC119730.1167061096861977No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA118870.11586782977030252No Hit
TAGTCCGGAACCGGATTGTTGTCATGAACTGCTGGGAGATCGGAAGAGCA115490.11257319475201683No Hit
ATCTGGAAGGTTTTCAGCTGCTGGAACGAGATCGGAAGAGCACACGTCTG113010.1101558294131563Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCTGAAAGGTCTGTATGCAACCCAGATCGGAAGAGCACACGTCTGAAC112300.10946376111049866Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCAGCCGTATCAGGACCAGACCTAGATCGGAAGAGCACACGTCTGAACT110740.10794316033282832Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGATCAGGTTTATCGTCAGCGTCGTAAACTGAGATCGGAAGAGCACAC107600.10488246389572266No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG104170.10153909167302444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)