FastQCFastQC Report
Fri 14 Jul 2023
SRR9691779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3523380
Total Bases267.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC776172.2029131118414704Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC297510.8443880591931611No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA287020.814615511242046Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA278840.7913991678445129No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG267910.7603778190260488No Hit
AGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGC255950.7264331409044724No Hit
TGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGT222140.6304741469838621No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT214020.6074280946136947Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG194680.552537620126129No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG188020.5336353160885287No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCAGATCGGAAGAGCACAC178790.5074388797120947No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA170740.4845915002071874No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA158060.4486033297572217No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC151860.43100659026275906No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA146180.4148857063387997Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCAGATCGGAAGAGCACA145240.412217813576736No Hit
ATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGTCT145220.4121610499009474Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC129700.3681124374890021No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC128530.3647917624553696No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA124520.3534106454597574Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG119500.33916296283682146No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAGATCGGAAGAGCACACGTC118690.33686403396738357No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA118040.3350192145042544No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG116420.3304213567653787No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCAGATCGGAAGAG114800.325823499026503No Hit
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACAC111690.3169967474413773No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCTGAGATCGGAAGAGCA106960.30357213811737593No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT105860.3004501359490035Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA104510.2966185878332737No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA101860.28909740079128565No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA96240.27314680789469203No Hit
AGATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGT93410.2651147477706067No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA85860.24368646016041415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC85360.24226736826569942No Hit
ATCCGGTTCAGCGTGAACTGGTTGATACCACCTATGCACGAGATCGGAAG84030.23849258382575822No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA80870.2295239230511611No Hit
TTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCAC75290.21368685750614463No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG69360.1968564276348279No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC65610.1862132384244674Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGTCT62940.17863528770669074Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT58290.1654377330858437No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT58210.16521067838268935Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC55820.15842741912595293Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC55730.15817198258490428Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC53610.1521550329513138Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG53050.15056565002923328No Hit
TTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACA52740.14968581305451018No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACTATCTCGTATG51840.14713144764402364TruSeq Adapter, Index 19 (97% over 37bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACAGATCGGAAGAGCACACG51320.1456555920735203No Hit
ATGCGTCCGGGTGGTCTGATTGCAACCGATAATGTTCTGTAGATCGGAAG48020.13628958556840307No Hit
AGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGTCTGAACTC47960.1361192945410373Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAAGATCGGAAGAGCACACGT47870.13586385799998865No Hit
ATGCGTCCGGGTGGTCTGATTGCAACCGATAATGTTCAGATCGGAAGAGC47620.1351543120526313No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAAGATCGGAAGAGCACAC46430.13177687334321023No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA45900.13027263593481261No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT44980.12766150684853747Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC43270.12280821256861309No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT42620.12096339310548394TruSeq Adapter, Index 3 (100% over 35bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC42320.12011193796865509Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACACGTCTG42230.11985650142760644Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG41870.11883475526341183Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA41370.11741566336869709Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA40790.115769516770828No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC40550.11508835266136493No Hit
ATACCACCATTACCGCAGAACTGAGATCGGAAGAGCACACGTCTGAACTC40450.11480453428242199Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA39550.11225016887193547Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT37790.1072549654025396TruSeq Adapter, Index 3 (100% over 35bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA37520.10648865577939366No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA37280.10580749166993059No Hit
TATGGATAGCGTTCTGCTGACCGTTAGTGATGGTCTGAGATCGGAAGAGC37270.10577910983203628No Hit
TATGCGTCCGGGTGGTCTGATTGCAACCGATAATGTTCAGATCGGAAGAG37040.10512632756046751No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT36100.10245843479840382Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)