FastQCFastQC Report
Fri 14 Jul 2023
SRR9691780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433947
Total Bases260.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC773812.253412763796296Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC286700.8348993155689356No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA285020.8300069861299547Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA274370.7989931120078441No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG256980.7483516781126791No Hit
AGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGC253960.7395571335259397No Hit
TGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGT224230.6529803750611177No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT210500.6129972303008754Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG187770.5468051778318069No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG177690.5174512011979218No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCAGATCGGAAGAGCACAC173540.5053659826432965No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA169230.49281482795162534No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA157000.45719983447618734No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC147020.42813706792795575No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA144790.42164308301788Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGTCT144630.42117714688083424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCAGATCGGAAGAGCACA141200.41118864094291496No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC129390.3767967298272221No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC125730.36613844069229956No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA123190.35874170451669757Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG119510.3480251733646442No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAGATCGGAAGAGCACACGTC117960.34351141703701304No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA115330.3358525917843228No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG113890.33165916655091066No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCAGATCGGAAGAG111050.32338880011834775No Hit
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACAC110830.3227481379299098No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCTGAGATCGGAAGAGCA106060.308857416844232No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT105870.30830411768149013Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA102910.2996842991461429No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA98280.28620127218038016No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA93180.27134955781204545No Hit
AGATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGT92930.2706215325979114No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC84440.24589779632591882No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA83730.2438302047177781Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCGGTTCAGCGTGAACTGGTTGATACCACCTATGCACGAGATCGGAAG81010.23590929038799957No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA78660.22906585337513946No Hit
TTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCAC74230.21616524658068398No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG67040.19522724142218853No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC64120.18672390692110274Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGTCT60100.17501726147782712Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT57740.16814470345640165Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC57090.1662518378996531Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT56400.16424248830864308No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC54830.15967048996388122Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACA53660.15626333196173384No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACAGATCGGAAGAGCACACG51000.14851714368334748No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG50970.1484297806576514No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACTATCTCGTATG50520.14711933527221008TruSeq Adapter, Index 19 (97% over 37bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC50440.1468863672036872Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGCGTCCGGGTGGTCTGATTGCAACCGATAATGTTCTGTAGATCGGAAG47680.1388489688396472No Hit
AGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGTCTGAACTC47390.1380044595912517Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGCGTCCGGGTGGTCTGATTGCAACCGATAATGTTCAGATCGGAAGAGC46010.1339857604092317No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAAGATCGGAAGAGCACACGT45790.13334509822079374No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA45050.13119014358695694No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT44820.1305203603899536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAAGATCGGAAGAGCACAC44460.12947200408160056No Hit
AGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACACGTCTG43220.12586099901949563Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT42940.1250456107796655TruSeq Adapter, Index 3 (100% over 35bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC41610.12117251664047232No Hit
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG41320.1203280073920768Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA40260.11724118048414842Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA40080.11671700232997188No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC39990.11645491325288364Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC39850.11604721913296856No Hit
ATACCACCATTACCGCAGAACTGAGATCGGAAGAGCACACGTCTGAACTC38480.11205764095951394Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT37640.1096114762400235TruSeq Adapter, Index 3 (100% over 35bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA37420.10897081405158554Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATGCGTCCGGGTGGTCTGATTGCAACCGATAATGTTCAGATCGGAAGAG36700.10687410143487945No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA36030.10492299386100018No Hit
TATGGATAGCGTTCTGCTGACCGTTAGTGATGGTCTGAGATCGGAAGAGC36020.10489387285243482No Hit
AAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCAC35070.1021273770387254No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT34480.10040923753336904Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)