FastQCFastQC Report
Fri 14 Jul 2023
SRR9691782_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691782_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3502662
Total Bases266.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC765202.18462415157386Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC288080.8224601745757941No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA285830.8160364888190753Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA272620.7783223159985176No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG263680.7527988712584885No Hit
AGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGC255490.7294166551040323No Hit
TGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGT222330.6347458019072352No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT210850.6019707296907323Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG190090.5427015224420741No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG183280.5232591668850719No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCAGATCGGAAGAGCACAC174190.4973074764279282No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA167370.4778365711564519No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA156590.44705997895315047No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC150020.42830281654353175No Hit
ATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGTCT142740.4075186244062373Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCAGATCGGAAGAGCACA142690.40737587583386575No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA141690.40452090438643523Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC130920.37377286189760817No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC126170.360211747522313No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA122430.34953415430892276Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG118740.33899930966790404No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAGATCGGAAGAGCACACGTC118600.3385996136652637No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA115830.33069134275588113No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG114100.32575224215182624No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCAGATCGGAAGAG110390.3151602980818589No Hit
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACAC110240.3147320523647443No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCTGAGATCGGAAGAGCA106000.3026269734276387No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT105980.3025698739986901Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA104850.2993437562630936No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA100700.28749562475625684No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA95480.2725926738006693No Hit
AGATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGT92480.26402775945837764No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA84010.23984615129864087Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC83530.23847576500387418No Hit
ATCCGGTTCAGCGTGAACTGGTTGATACCACCTATGCACGAGATCGGAAG82720.23616323813145543No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA81290.23208062896162973No Hit
TTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCAC74990.21409430884281724No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG69660.1988773110280124No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC64460.18403145950137353Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGTCT61960.17689403088279712Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT58620.1673584262483791Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC56740.16199107992720965Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT55990.15984985134163673No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC55500.15845091533239578Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACA52470.14980035184668117No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG51730.14768767297558258No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACTATCTCGTATG51330.14654568439661037TruSeq Adapter, Index 19 (97% over 37bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC50780.14497545010052354Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACAGATCGGAAGAGCACACG49310.14077864207280064No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAAGATCGGAAGAGCACACGT47790.1364390854727062No Hit
ATGCGTCCGGGTGGTCTGATTGCAACCGATAATGTTCAGATCGGAAGAGC47170.13466900317529923No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA47100.13446915517397912No Hit
ATGCGTCCGGGTGGTCTGATTGCAACCGATAATGTTCTGTAGATCGGAAG46900.133898160884493No Hit
AGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGTCTGAACTC46500.13275617230552078Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT45540.13001539971598744Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAAGATCGGAAGAGCACAC44680.12756012427119717No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC43200.12333476652899994No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT42480.12127918708684994TruSeq Adapter, Index 3 (100% over 35bp)
AGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACACGTCTG42030.11999444993550619Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG41270.11782467163545898Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA40930.11685398134333258No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC40720.11625443733937217Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA40510.11565489333541176Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC40210.11479840190118258No Hit
ATACCACCATTACCGCAGAACTGAGATCGGAAGAGCACACGTCTGAACTC39590.11302831960377566Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA38270.10925975729316731No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT38020.10854601443130968TruSeq Adapter, Index 3 (100% over 35bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA37960.10837471614446384Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATGGATAGCGTTCTGCTGACCGTTAGTGATGGTCTGAGATCGGAAGAGC37420.10683303156285134No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA36620.10454905440490689No Hit
TATGCGTCCGGGTGGTCTGATTGCAACCGATAATGTTCAGATCGGAAGAG36590.10446340526148398No Hit
AAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCAC35440.10118018809693885No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)