FastQCFastQC Report
Fri 14 Jul 2023
SRR9691785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4296427
Total Bases326.5 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT749741.7450313946914493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC526071.224436025562636Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA407890.9493702557962699No Hit
AGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGC336200.7825106768950106No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA334610.7788099274117772No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC279930.6515413854349207No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG278400.647980286875583No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC243240.5661448454727614No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCAGATCGGAAGAGCACAC236870.5513185723858453No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG231330.5384241370794849No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCAGATCGGAAGAG206620.48091123158848037No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA206290.4801431515070546Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA204020.4748596915530044Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG178550.4155778743593223No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG160550.37368259719064234No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG136860.31854375740586305No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA134560.31319047198986505No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA127570.2969211393560277No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA124200.2890774124638915No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA117170.27271497921412374Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGAGCACAC116730.27169087243888934No Hit
AGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAGCACACGTC114890.26740824410609093No Hit
AGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACACGTCTG112050.2607981003750326Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCTAATTACTGAAGAGACTACTCATGAGATCGGAAGAGCACACGTCTGA110720.2577025048953468Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA104390.242969332424361No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG104260.24266675542258717No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA103090.23994356240662298No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTAGATCGGAAGA101850.2370574433127806No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT100260.2333566938295472Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCTGAGATCGGAAGAGCA99420.23140158089500878No Hit
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACAC97200.22623449671087165No Hit
TGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGT97100.2260017451710456No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAACTATCTCGTATG94770.22057863429309982TruSeq Adapter, Index 2 (97% over 36bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAAGATCGGAAGAGCACACGT94360.2196243529798132No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG94100.21901919897626562No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA93330.21722701211960546Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGT91310.21252543101512025No Hit
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC89870.20917380884162584No Hit
ATGCTGAGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACA87800.20435585196722766No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACAGATCGGAAGAGCACACG85420.19881636531936886No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC84020.19555784376180485No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC83940.19537164252994405No Hit
ATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGAGATCGGAAGAGC82500.1920200203564497No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG82250.19143814150688465No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAGATCGGAAGAGCACACGTC81060.18866839818295528No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA80680.18778394233161647No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT78590.1829194351492531Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCAGATCGGAAGAGCACA77750.1809643222147147No Hit
TTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCAC70600.16432258711715572No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC69590.16197179656491312No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG68050.15838742285159274No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT67820.15785209430999292No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA66380.15450047213649853No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG66330.15438409636658554No Hit
ACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGC66020.15366256659312494No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC65680.15287121135771656No Hit
ATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGTCT63870.14865840848686593Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTCCGGGTGGTCTGATTGCAACCGATAATGTTCTGTTTCAGATCGG63770.14842565694703994No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA59740.13904576989205217Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG58300.13569414771855776TruSeq Adapter, Index 11 (100% over 35bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC57940.1348562421751842Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAG57920.13480969186721897No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA57910.13478641671323638Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA55570.12934003068130798Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA53830.1252901538883356No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC52000.1210308007095198Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATGCTGAGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCA51200.1191687883909118No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG50730.11807485615372959No Hit
ATCTAATTACTGAAGAGACTACTCATGTTAGATCGGAAGAGCACACGTCT50390.11728350091832121Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAGCA49990.11635249475901721No Hit
ATCCGGTTCAGCGTGAACTGGTTGATACCACCTATGCACGAGATCGGAAG49840.1160033674492782No Hit
AGATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGT49750.1157938910634348No Hit
TTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACA49630.1155145892156436No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG48840.11367585205101821Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA47580.11074318264921061No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC46120.10734501016775103Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC45640.10622780277658621Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA45450.10578557485091682No Hit
ATGCGTCCGGGTGGTCTGATTGCAACCGATAATGTTCTGTTAGATCGGAA45190.10518042084736923No Hit
AACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCTG44820.10431924015001304Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)