FastQCFastQC Report
Fri 14 Jul 2023
SRR9691786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4192119
Total Bases318.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT735371.75417253183891Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC511841.2209577065918213Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA398630.950903349833342No Hit
AGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGC326000.7776496802690954No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA324360.7737375775830792No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC276110.6586406540463189No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG273760.6530348971486735No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC235400.5615298611513653No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCAGATCGGAAGAGCACAC231060.5511771016042245No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG224250.5349323337433886No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCAGATCGGAAGAG200820.47904174475963107No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA199710.47639391916116886Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA198460.473412133577315Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG176000.41983541020662823No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG157680.37613436068966555No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG135470.32315399443574955No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA131240.313063632019988No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA122720.29273978148043983No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA122390.2919525900863024No Hit
AGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAGCACACGTC113100.2697919596271003No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA112590.2685753911088879Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGAGCACAC110990.2647587055615549No Hit
AGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACACGTCTG108300.2583419029851013Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCTAATTACTGAAGAGACTACTCATGAGATCGGAAGAGCACACGTCTGA106800.25476376028447667Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA101400.24188244656222785No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG100560.23987868664987802No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA100070.23870982670100732No Hit
TGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTAGATCGGAAGA99810.23808961529956568No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCTGAGATCGGAAGAGCA98450.23484543258433266No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT97310.2321260441318579Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGT96230.22954978138740814No Hit
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACAC96080.22919196711734566No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAAGATCGGAAGAGCACACGT92620.2209383846212381No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA91870.21914931327092577Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG91490.21824285045343417No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAACTATCTCGTATG90820.2166446133804885TruSeq Adapter, Index 2 (97% over 36bp)
AAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGT88670.2115159421762598No Hit
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC86290.205838622424602No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACAGATCGGAAGAGCACACG85360.20362017395021467No Hit
ATGCTGAGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACA83790.19987505125689417No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC82870.1976804570671777No Hit
ATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGAGATCGGAAGAGC81310.19395918865852807No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG78980.18840114033022443No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA78460.18716071752734117No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC77860.18572946044709132No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGAGATCGGAAGAGCACACGTC77360.18453674621354976No Hit
AGCGTTCTGCTGACCGTTAGTGATGGTCTGACCCAGATCGGAAGAGCACA77060.18382111767342482No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT75360.17976588927938353Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCAC70610.16843510406073875No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC68070.16237611575434763No Hit
ACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGC66450.15851172163767296No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG66130.15774838452820636No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT65510.15626941887861484No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA65400.15600702174723569No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG64370.1535500304261401No Hit
ATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGTCT64210.1531683618714068Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC62400.14885073634598636No Hit
ATGCGTCCGGGTGGTCTGATTGCAACCGATAATGTTCTGTTTCAGATCGG59440.14178986808342034No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA59060.14088340526592877Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG57760.1377823482587207TruSeq Adapter, Index 11 (100% over 35bp)
AGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAG57480.13711442828793743No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA56770.13542077407630843Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC54290.1295049114779423Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA53140.12676166874079672Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA51090.12187154038327634No Hit
AGATGCTGAGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCA50160.11965309190888904No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG49670.11848423196001831No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC49380.1177924577045642Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTAGATCGGAAGAGCA49310.11762547771186839No Hit
ATCCGGTTCAGCGTGAACTGGTTGATACCACCTATGCACGAGATCGGAAG48880.11659974347102266No Hit
AGATGCTGAGTTTGTGTGTGAACGGACACTGAGATCGGAAGAGCACACGT48080.11469140069735617No Hit
TTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACA48070.11466754641268533No Hit
ATCTAATTACTGAAGAGACTACTCATGTTAGATCGGAAGAGCACACGTCT47800.11402348072657288Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG47720.11383264644920622Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA47070.11228211794560221No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC44620.10643781820124858Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA43860.10462489256626542No Hit
AACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCTG43850.10460103828159459Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC42930.10240644409187812Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)