FastQCFastQC Report
Fri 14 Jul 2023
SRR9691787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6135260
Total Bases466.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC1262542.0578426994129018Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC1243002.025994008403882No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT712761.1617437565808133Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA675371.100800944051271Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA457420.7455592754015316No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA353690.57648738602765No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA333550.5436607413540746Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT322190.5251448186384929No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG317760.5179242607485257No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA296770.4837121817168302No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG294000.479197295632133No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC245510.40016234030831616No Hit
AGACTGCTGCTGGAACTGGGCACCTACTGCGGCTACAGCGAGATCGGAAG230960.3764469639428484No Hit
AGACTGCTGCTGGAACTGGGCACCTACTGCGGCTACAGCGCCCTGAGATC199220.3247132150878691No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT188300.30691445839296133Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA178780.2913975935820161No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG166170.27084426739861067No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC154530.25187196630623643No Hit
ACTGGACTTCGTGTTCGTGGACCACGAGATCGGAAGAGCACACGTCTGAA152540.2486284199854611Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT149520.24370605320719907No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA145480.23712116519919285No Hit
ACGACGGCCGGACCATCGAGAGACTGAGATCGGAAGAGCACACGTCTGAA135050.22012107066367195Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC134910.21989288147527572No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT129970.21184106297043645No Hit
TGGACCGGCGGTGGCTGCACCCTGGATCTGTGGTGCTGGCCGAGATCGGA127990.20861381587740374No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG124050.20219192014682344No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG113240.18457245495708413Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC112370.18315442214347882No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG107570.17533079282703584No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA106340.17332598781469732Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCCACCCTGATCAACATCGGCGACGAGATCGGAAGAGCACACGTCTGA103270.16832212489772236Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT95120.15503825428751186Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT92630.15097974657960705Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC90350.14726352265429662Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTGGACTTCGTGTTCGTGGACCAGATCGGAAGAGCACACGTCTGAACTC88610.14442745702708606Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA86590.14113501302308296Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC82640.13469681806476008No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA79430.12946476595938886No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC76170.12415121771530466No Hit
TTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCC75110.12242349957459016Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG71210.11606680075498023No Hit
TCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCCA70140.11432278338652316Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT68560.11174750540319399TruSeq Adapter, Index 12 (100% over 35bp)
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC67430.10990569266828137Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC66640.1086180536766168No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC66030.10762380078431884No Hit
TTTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT64240.10470623901839532Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC63510.10351639539318627No Hit
ACGACGGCCGGACCATCGAGAGACTGACAACCAGATCGGAAGAGCACACG63420.10336970234350296No Hit
AGAGACTGACAACCAGACACGGCTTCGGCCCTGGCAGATCGGAAGAGCAC62500.10187017339118472No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG61890.10087592049888676No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)