FastQCFastQC Report
Fri 14 Jul 2023
SRR9691788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5865466
Total Bases445.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC1239502.113216579893226Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC1197032.040809715715682No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT682991.1644258103277727Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA659131.1237470305002195Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA440370.750784336657991No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA333220.5681049041968703No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA327770.5588132298439715Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG309720.5280398863449213No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT308950.5267271176748787No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA287500.49015713329512095No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG281230.4794674455533456No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC234330.3995078992871155No Hit
AGACTGCTGCTGGAACTGGGCACCTACTGCGGCTACAGCGAGATCGGAAG217390.3706269885461786No Hit
AGACTGCTGCTGGAACTGGGCACCTACTGCGGCTACAGCGCCCTGAGATC188930.3221056945859033No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT187120.3190198357641149Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA170900.2913664489743867No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG162940.27779548973602436No Hit
ACTGGACTTCGTGTTCGTGGACCACGAGATCGGAAGAGCACACGTCTGAA149680.25518859030126506Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC145340.24778934870647956No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT141450.24115730958119952No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA139810.23836128280344646No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC130660.22276149925683653No Hit
ACGACGGCCGGACCATCGAGAGACTGAGATCGGAAGAGCACACGTCTGAA130050.22172151368706253Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT127400.21720354358886404No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG121680.207451547754262No Hit
TGGACCGGCGGTGGCTGCACCCTGGATCTGTGGTGCTGGCCGAGATCGGA119170.2031722628687985No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG109290.1863279064272131Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC109110.18602102543941096No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG105810.1803948739963713No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA102420.17461528205943058Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCCACCCTGATCAACATCGGCGACGAGATCGGAAGAGCACACGTCTGA100660.1716146679564761Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT90910.15499194778385894Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT89610.15277558509417666Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC86430.14735402097633846Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA85210.14527404983679046Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGGACTTCGTGTTCGTGGACCAGATCGGAAGAGCACACGTCTGAACTC84500.1440635748293486Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC79140.13492534097035086No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA74020.126196281761756No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC73720.1256848134487524No Hit
TTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCC72890.12426975111610909Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCCA69430.11837081657280087Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC66920.11409153168733738Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT66170.11281286090482835TruSeq Adapter, Index 12 (100% over 35bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG65810.11219909892922404No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC63050.10749359044959086No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC62320.10624901755461544No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC62150.10595918551058005Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT62040.1057716471291454Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGACGGCCGGACCATCGAGAGACTGACAACCAGATCGGAAGAGCACACG61640.10508968937847393No Hit
AGAGACTGACAACCAGACACGGCTTCGGCCCTGGCAGATCGGAAGAGCAC61600.10502149360340678No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC60630.10336774605802847No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA59350.10118548125587976No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG59120.10079335554924365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)