FastQCFastQC Report
Fri 14 Jul 2023
SRR9691789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5583142
Total Bases424.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC1168202.092370210179143Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC1135302.03344281768223No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT651371.166672816131132Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA640241.146737804626857Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA420360.7529093832827466No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA316750.5673328745713435No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA311290.5575534349654729Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT298670.5349496752903651No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG297230.5323704824272784No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA276060.49445276512759306No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG269630.4829359525514486No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC225550.40398399324251466No Hit
AGACTGCTGCTGGAACTGGGCACCTACTGCGGCTACAGCGAGATCGGAAG207310.37131421697674893No Hit
AGACTGCTGCTGGAACTGGGCACCTACTGCGGCTACAGCGCCCTGAGATC179470.32144982162373803No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT178590.3198736482074072Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA164360.29438620762287615No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG154010.27584825891944No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT138320.2477458033487237No Hit
ACTGGACTTCGTGTTCGTGGACCACGAGATCGGAAGAGCACACGTCTGAA138280.24767415910252685Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC137410.24611589674774526No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA130430.2336139757863941No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC126500.22657492859755315No Hit
ACGACGGCCGGACCATCGAGAGACTGAGATCGGAAGAGCACACGTCTGAA122630.21964334777800745Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT120820.21640144563759975No Hit
TGGACCGGCGGTGGCTGCACCCTGGATCTGTGGTGCTGGCCGAGATCGGA116660.20895044403312688No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG114180.20450850076892188No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG106030.18991098560631273Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC104320.1868481940813972No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG100770.18048976723142632No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA97840.1752418261975067Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCCACCCTGATCAACATCGGCGACGAGATCGGAAGAGCACACGTCTGA94970.1701013515328824Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT84970.1521902899836687Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT83950.1503633617056489Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC83040.14873345510467045Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA80550.14427360077891624Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGGACTTCGTGTTCGTGGACCAGATCGGAAGAGCACACGTCTGAACTC80210.14366462468624297Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC76350.13675095492824652No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA70560.12638045029125178No Hit
TTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCC69900.12519832022900365Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC69570.12460725519787962No Hit
TCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCCA63940.11452332754567231Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG63400.11355613022201477No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT63050.11292924306779228TruSeq Adapter, Index 12 (100% over 35bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC62250.1114963581438552No Hit
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC61980.11101275948202642Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC60490.10834401131119357No Hit
ACGACGGCCGGACCATCGAGAGACTGACAACCAGATCGGAAGAGCACACG58910.10551406358641784No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC58140.10413491184712838No Hit
AGAGACTGACAACCAGACACGGCTTCGGCCCTGGCAGATCGGAAGAGCAC58050.10397371229318546No Hit
TTTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT57850.10361549106220116Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC57160.10237962781530544Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG56080.10044523316799034No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)