FastQCFastQC Report
Fri 14 Jul 2023
SRR9691793_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691793_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5443389
Total Bases413.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC1278402.34853691330897Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA993881.8258478311948676Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG606211.1136628302698925No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC571961.050742469443209No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT445910.819177170692743Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA433750.7968381462357366No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA423120.7773098707441265No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA339690.6240413830428065Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT319200.5863993919964199No Hit
AGACTGCTGCTGGAACTGGGCACCTACTGCGGCTACAGCGAGATCGGAAG288820.5305885726704448No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC274360.5040242393112084No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG265730.4881701454737113Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA248740.4569579723220221No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC238140.4374848095552238No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT223930.41137974890275164Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG174130.3198926257153402No Hit
TGGACCGGCGGTGGCTGCACCCTGGATCTGTGGTGCTGGCCGAGATCGGA171860.315722429537922No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG156860.28816606713207527No Hit
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC131530.2416325564827353Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT128250.23560689856999012No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT126690.23274103687978207Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGAGTGGCGGACCAGAGAACACCACGCCAGATCGGAAGAGCACACGTC125280.23015073881363246No Hit
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT118150.21705228121671996Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA118020.2168134594092026Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC114690.21069594695510463No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT114430.21021830334006994Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA113660.20880374340323649No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC109820.2017493146273397No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCTCGTATG107840.1981118747897679TruSeq Adapter, Index 10 (97% over 38bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG106900.19638500941233486No Hit
TTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCC104600.192159700510105Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA103240.18966125698530822Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC100310.18427858086203283Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGA99470.1827354245673054Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCCA97210.1785835992981578Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA96770.17777527933425297Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA95980.17632397758087837Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACTGTGCAGCGTGGAATTCAGCGCCGAGATCGGAAGAGCACACGTCTG95010.1745419994786336Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG85900.15780610204414933No Hit
AGACTGCTGCTGGAACTGGGCACCTACTGCGGCTACAGCGCCAGATCGGA84200.15468304763815338No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAA82010.15065981872689974TruSeq Adapter, Index 7 (97% over 35bp)
ACGACGGCCGGACCATCGAGAGACTGAGATCGGAAGAGCACACGTCTGAA81530.14977801512991265Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT80840.14851042245924367Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGGACTTCGTGTTCGTGGACCAGATCGGAAGAGCACACGTCTGAACTC78810.1447811280803191Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACTGCTGCTGGAACTGGGCACCTACTGCGGCTACAGCGCCCTGAGATC78320.14388095357506142No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC74700.13723068478111708Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC70610.12971698329845616Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC69300.1273103943150122Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC68400.1256570125706614No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA65380.1201089982729509Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCAGCTGTCTTCTGACGTTCAGACTCAGATCGGAAGAGCACACGTCTGA63320.11632459116921463Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA61890.1136975512865239Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC61860.1136424385617122Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA61440.11287086041434848Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGAGAGACTGACAACCAGACACGGCTTCGGCCCTGGCAGATCGGAAGAG59870.10998662781586985No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA59250.10884763150309484No Hit
TGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGAGCACACGTC57890.10634918797829808No Hit
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC56040.10295056994824364No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)