FastQCFastQC Report
Fri 14 Jul 2023
SRR9691794_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691794_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5180632
Total Bases393.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC1206452.3287699261402857Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA948231.8303365303692678Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG569261.0988234640097965No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC540541.0433862123385718No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT427000.8242237626606175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA412750.7967174661315455No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA403700.7792485550025556No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA327780.6327027281613518Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT305680.5900438402110013No Hit
AGACTGCTGCTGGAACTGGGCACCTACTGCGGCTACAGCGAGATCGGAAG270810.522735450037756No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC261290.5043593136899127No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG253870.4900367368305643Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA234880.45338097745603234No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC224280.43292015337124895No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT211770.4087725204183582Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG165340.3191502503941604No Hit
TGGACCGGCGGTGGCTGCACCCTGGATCTGTGGTGCTGGCCGAGATCGGA164120.3167953253579872No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG150920.29131580857316247No Hit
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC125430.2421133174485275Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT122120.2357241355880904No Hit
ACCGAGTGGCGGACCAGAGAACACCACGCCAGATCGGAAGAGCACACGTC118830.22937355905611514No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT118220.22819609653802858Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA113400.2188922123787214Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT111010.2142788756275296Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT109550.21146068664981416Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC109330.21103602803673374No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA105200.20306402770936058No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG105070.2028130930743585No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC104980.20263936909628014No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCTCGTATG102970.19875953358586365TruSeq Adapter, Index 10 (97% over 38bp)
TTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCC98970.19103846789349255Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA98700.19051729595925748Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCCA93420.1803254892453276Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGA92940.17939896136224306Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC92930.17937965869801212Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA92290.17814428818723277Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACTGTGCAGCGTGGAATTCAGCGCCGAGATCGGAAGAGCACACGTCTG92030.17764241891722862Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA90690.17505586191028433Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGACTGCTGCTGGAACTGGGCACCTACTGCGGCTACAGCGCCAGATCGGA81490.15729741081783072No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG79960.15434410319049877No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAA78970.15243313943163692TruSeq Adapter, Index 7 (97% over 35bp)
ACGACGGCCGGACCATCGAGAGACTGAGATCGGAAGAGCACACGTCTGAA77600.14978867443199978Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT77190.14899726519853176Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGGACTTCGTGTTCGTGGACCAGATCGGAAGAGCACACGTCTGAACTC72440.13982849968884103Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACTGCTGCTGGAACTGGGCACCTACTGCGGCTACAGCGCCCTGAGATC71920.1388247611488328No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC71160.13735775866728228Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC67930.1311229981206926Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC66020.1274361892525854No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC65760.12693431998258128Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA62730.12108561272061015Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC59640.11512108947325346Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA59110.11409804826901429Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA58650.11321012571439161Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCAGATCGGAAGAGCACACGTCTGA58110.11216778184592151Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA57670.11131846461976068No Hit
TCGAGAGACTGACAACCAGACACGGCTTCGGCCCTGGCAGATCGGAAGAG56710.1094654088535916No Hit
TGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGAGCACACGTC55050.10626116659125759No Hit
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC53970.10417647885431738No Hit
AGAGACTGACAACCAGACACGGCTTCGGCCCTGGCAGATCGGAAGAGCAC52020.10041245932928645No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)