FastQCFastQC Report
Fri 14 Jul 2023
SRR9691795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3588737
Total Bases272.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGAGATCGGAAGAGCACAC1432313.9911255686889286No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA621561.7319742293737321Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTGATCGTGTTGGTACATATACCGAAGCAGATCGGAAGAGCACACGT521321.4526559065208735No Hit
ATGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGAGATCGGAAGAGCACA514551.4337913310448773No Hit
TGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGAAGATCGGAAGAGCACA330140.9199336702578094No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA255930.7131478288879903No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC234590.6536840119518371No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG230910.6434297080003355No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC202610.5645718814167769No Hit
ATGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGAAGATCGGAAGAGCAC169450.47217168602770276No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA145500.40543511547377253Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA143540.3999735840213423No Hit
AGATCGTGATCGTGTTGGTACATATACCGAAGCAGATCGGAAGAGCACAC132120.36815180382401946No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG129330.36037748099122335No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG128750.3587613135206063No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA123310.34360277724447347No Hit
AGCAGCCGATGGTCGTCAGGCAGAAGATCGGAAGAGCACACGTCTGAACT120330.33529902024026836Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGATAGATCGGAAGAGCAC111950.3119481867854903No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG101190.2819654937099041Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGATCGTGTTGGTACATA99230.27650396225747387No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA97700.2722406239298115No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGA93430.26034228755130284No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCA90930.2533760484538154No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA87280.24320533937148364No Hit
ATCGTGATCGTGTTGGTACATATACCGAAGCAAGATCGGAAGAGCACACG87030.24250871546173486No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA85180.2373536985295941No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA80400.224034249375198Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG80020.2229753810323799No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG78520.2187956375738874No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA76770.2139192702056462Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC75960.21166220873806021Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGATAGATCGGAAGAGCA72170.2011013902662692No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA69240.1929369580440138Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG65410.18226467974666297No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG56120.15637813526039943No Hit
TGTTCGTCTGCCAGGTGAAGCAGCCGATGGTCGTCAGGCAGAAGATCGGA55370.15428826353115316No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC54340.15141817302298832No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA54100.15074941406962952No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA53050.14782359364868475No Hit
TGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGATCAGATCGGAAGAGCA50660.1411638690714867No Hit
ATCGTGATCGTGTTGGTACATATACCGAAGCAAGCAAGATCGGAAGAGCA49860.1389346725602907No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC47730.13299943684923135No Hit
TGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGATCGTGTTGGTACATAT43950.12246648333383026No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA42790.11923414839259605No Hit
ATCGTGATCGTGTTGGTACATATACCGAGATCGGAAGAGCACACGTCTGA42120.1173671963144694Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC41710.11622473310248145Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGA40760.1135775622454362No Hit
TGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGATCGTGTTGGTACAGAT40610.11315958789958695No Hit
AAGCAGCCGATGGTCGTCAGGCAGAAGATCGGAAGAGCACACGTCTGAAC38820.10817176070578591Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGATCGTGTTGGTACAAG38440.10711289236296781No Hit
TGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGATCGTGTTGGTACAAGA38100.1061654838457095No Hit
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC37560.10466077620065221Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)