FastQCFastQC Report
Fri 14 Jul 2023
SRR9691800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4578218
Total Bases347.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGAGATCGGAAGAGCACAC2735045.974027449107928No Hit
ATCGTGATCGTGTTGGTACATATACCGAAGCAGATCGGAAGAGCACACGT976192.132248835682355No Hit
ATGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGAGATCGGAAGAGCACA932112.0359668325099416No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA498921.0897689887200654Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC463791.0130360764821595No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATCTCGTATG369260.8065583596062922TruSeq Adapter, Index 10 (97% over 36bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA364650.796488939583043No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG310380.6779493680729052No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA305190.6666130795868611Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGAAGATCGGAAGAGCACA268680.5868658941098917No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA215480.47066347648801343Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA215350.47037952321186977No Hit
AGATCGTGATCGTGTTGGTACATATACCGAAGCAGATCGGAAGAGCACAC174320.3807595007489814No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG168050.3670642158149743No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC158730.3467069501714422No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA121820.2660860623063384Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGAAGATCGGAAGAGCAC119700.26145543964922596No Hit
ATCGTGATCGTGTTGGTACATATACCGAGATCGGAAGAGCACACGTCTGA110320.24096711864747375Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG102340.223536756004192No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC96770.21137045024942017Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG94130.205604014487733TruSeq Adapter, Index 4 (100% over 35bp)
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA90910.1985707102632509Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA81180.17731789967188105No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA80630.17611655888819625No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA78520.17150777879078716Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC71880.15700431914775576No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC64110.14003265025824457Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA63730.13920263298951688No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT63530.13876578179544968Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA62310.13610098951163968No Hit
TGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGATAGATCGGAAGAGCAC61630.13461569545181118No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG60980.13319592907109273No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT60170.13142668173512054No Hit
ATCGTGATCGTGTTGGTACATAAGATCGGAAGAGCACACGTCTGAACTCC59940.13092430286194323Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAGATCGGAA55820.12192516826415867No Hit
TGCTGGCTCGTCTGACCAGCCGTGCAGATCGTGATCAGATCGGAAGAGCA55570.12137910427157467No Hit
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGG55220.12061461468195703TruSeq Adapter, Index 10 (97% over 36bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA55110.12037434652522007No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT54060.1180808777563672Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG53520.11690137953238576No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA51840.11323182950222117No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC51510.11251102503201027Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC50790.11093836073336831Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG50720.11078546281544478Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG50500.11030492650197085No Hit
AGCAGCCGATGGTCGTCAGGCAGAAATTCAGCGTCGTCTGGCCGAGATCG50150.10954043691235324No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC48950.10691932974794997Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC48360.1056306187254517Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG47340.10340267763570893Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG47090.1028566136431249No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG46180.10086894071011909No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA46170.10084709815041573No Hit
ATCGTGATCGTGTTGGTACATATACCGAAGCAAGATCGGAAGAGCACACG45890.10023550647872163No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)