FastQCFastQC Report
Fri 14 Jul 2023
SRR9691804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3393377
Total Bases257.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA742542.1882036684989616No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG353931.0430022953535667No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC345091.016951550034081No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA311510.9179940808227322Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG264370.7790764185647513No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA207850.6125166758659589No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA180610.5322426597457341No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA177750.5238144774364888No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC173310.5107301664389191No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG157490.46410994121784876No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG134860.39742121196672225No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC130370.38418955512458536No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA115580.34060465430159986Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA95560.2816073781368825No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG95350.28098852558970017No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG92780.27341494917894477No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA85880.25308122262866756No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA84610.24933863817666No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA83000.2445941019815953Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC79880.2353997212806004No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA79690.23483980707124497Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCA75660.22296373199912653No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC73210.21574378561533245Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA72740.21435873467640054No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA69890.20596002153606863No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA67220.1980917534361788No Hit
TTGTTCGTCGTCTGCAGGCAGCATCACCGGGTCTGACCCAGGGTGCAGTT62990.18562629498579145No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA62370.18379920651315784Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT53270.15698226280192268No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA53070.1563928794236538No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGA52340.1542416300929723No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA49230.14507671856089083No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA49210.14501778022306394No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA49020.14445786601370847No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG47180.13903553893363454No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC45810.13499826279249255No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG45440.13390790354269508No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG45390.13376055769812784Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC44910.13234603759028246Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA43000.12671742632781446No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA41590.12256227351101867No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG41560.12247386600427834No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG40230.11855446653879012No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCA39860.11746410728899265No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG39350.11596117967440694No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC38720.11410462203285988No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG38280.11280797860066831No Hit
AGTTGTTCGTCGTCTGCAGGCAGCATCACCGGGTCTGACCCAGGGTGCAG38220.11263116358718762No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT37740.11121664347934225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGATCGGAAGAGCA37540.11062726010107335No Hit
ACAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAGATCGGAAGAGCA34510.10169810192029946No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)