FastQCFastQC Report
Fri 14 Jul 2023
SRR9691806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503507
Total Bases266.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA760872.171738204033844No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG368431.0516034362140565No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC358151.0222614083545432No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA313490.8947891355718712Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG274320.7829868757219551No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA214000.6108165332622426No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA185170.5285275582437826No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA184900.5277569018700405No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC178850.5104884905324865No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG158630.45277489098780166No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG139050.3968880324771722No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC132790.3790202217378187No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA119250.34037323173608613Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA99300.28343028856514346No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG97460.27817840809223443No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG96820.2763516670581791No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA86710.24749486728583675No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA83670.23881784737407405Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA83530.23841824777287446Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA82940.2367342208821047No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC82570.23567813622179146No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA79590.22717237328197143No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCA76080.2171538404233244No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC73910.21096004660473064Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA69500.1983726591669433No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA68650.1959465187310886No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA67040.19135112331729323Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGTTCGTCGTCTGCAGGCAGCATCACCGGGTCTGACCCAGGGTGCAGTT62580.1786210217362203No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA55460.15829852773235503No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT55110.15729952872935604No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGA54380.1552159022373867No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA52450.14970713630656368No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA50360.14374168511722682No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA49940.14254288631362805No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG48430.1382329191864038No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC47230.1348077797475501No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC46200.13186786839586734Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG46010.13132555465138218No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA45190.12898504270149883No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA44250.1263020168077301No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG44140.12598804569250183Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG43240.12341919111336155No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG43030.12281979171156215No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC41940.11970862338793672No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCA41500.11845273892702371No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG40390.11528448494608404No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG38980.11125994610543093No Hit
AGTTGTTCGTCGTCTGCAGGCAGCATCACCGGGTCTGACCCAGGGTGCAG38910.11106014630483114No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT38160.10891943415554757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGATCGGAAGAGCA37450.10689289332089247No Hit
ACAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAGATCGGAAGAGCA35270.1006705566736416No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)