FastQCFastQC Report
Fri 14 Jul 2023
SRR9691808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4121476
Total Bases313.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA742941.8026066389807922No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA499411.2117260903618023No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC400620.9720304085235485No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC390250.946869519560468No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG321650.7804242945973724No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG313550.7607711412125171No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA243070.5897644436119487Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA203340.4933669394168497Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA155200.3765641241147589Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG148820.36108423293014447No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA117130.28419430320593886Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG99230.2407632605406413No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG92870.2253318956606808No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA90770.2202366336720146Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT89990.21834410779050997No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA88500.2147288980937897No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG86210.20917263621091087No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA86130.208978530992295No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA86020.2087116363166982No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT84070.20398032161293672No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC83890.20354358487105106Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA77610.18830632520970642No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA75460.18308974745940532No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA74930.18180380038607527No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC73280.17780038025212327Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA65490.15889938458940436No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC63040.1529549122692938Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA62160.1508197548645194No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG60950.1478839134329546No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC55460.13456344280544155No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA54550.1323554959436862Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTGTATGGACTGACGAAGAATCAGATCGGAAGAGCACACGTCTGAAC52070.12633823416659468Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA51450.1248339187223218No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT51340.124567024046725Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC49410.11988423564761751No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA47960.11636607856020513No Hit
CCCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA47500.11524997355316396No Hit
ACAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAGATCGGAAGAGCA47440.11510439463920208No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG44980.10913565916676454No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG43950.1066365544770854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT41710.10120160835584144No Hit
ATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAGATCGGAAGAGCACA41450.10057076639533992No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)