FastQCFastQC Report
Fri 14 Jul 2023
SRR9691813_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691813_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3709426
Total Bases281.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA540081.4559665026341004No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG399781.0777408688028822No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG326260.8795430883376566No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC318350.858219034427429No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA201440.5430489784672885Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC178900.48228486024522393No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG162340.4376418346126867No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA141930.38261984468756083Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG140220.37800996704072276No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC133980.36118795738208553Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA123990.3342565669189788No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA104490.2816877867357375Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA102860.2772935758793948No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA101060.27244107309324944No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA100200.27012265509542444No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA99530.26831644572502594No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC94720.2553494799464931Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC92460.24925689311499943Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC91500.24666889162905528Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC87370.2355350935697329No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG77890.20997857889603405No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA76920.20736361906127793No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG76420.206015701620682No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC74130.19984223974275264Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG73880.1991682810224547No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT72520.19550194558403375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC72360.19507061200304307No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCACATCTCGTATG69970.1886275666369945TruSeq Adapter, Index 11 (97% over 49bp)
ATCCGAAAGTTACCTGCAAGCAAGAATGAGATCGGAAGAGCACACGTCTG69830.18825014975362764Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG66050.17805989390272242No Hit
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAGATCGGAAGAGCACACG64420.17366568304637967No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC63140.1702150143984541No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA60760.16379892738121746No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG58070.15654713155081137No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA56460.15220683739209248No Hit
AGCTCTTGATCCGGCAAACAAACCACCGCTAGATCGGAAGAGCACACGTC55060.14843266855842388No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA53010.14290620705198057No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG52220.14077649749583898No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA51080.13770324573128023No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA50230.13541178608226717Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT49740.13409082699048316No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC48940.13193415908552966No Hit
ATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGCAGATCGGAAGA47900.12913049080909014No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG45220.12190565332749596TruSeq Adapter, Index 11 (100% over 35bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT43640.11764623421521281Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA43580.11748448412234129Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA42730.11519302447332823No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA42230.11384510703273229Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA41600.11214673105758141No Hit
AACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCTG39260.10583847743559245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC37260.10044680767320874No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)