FastQCFastQC Report
Tue 18 Jul 2023
SRR9693936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130770594
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG20235591.5474113392801443Illumina RNA RT Primer (100% over 22bp)
TCCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG16549241.2655169250053266Illumina RNA RT Primer (100% over 22bp)
CCGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG13668371.0452173980336894Illumina RNA RT Primer (100% over 22bp)
AGCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG13555761.036606134862399Illumina RNA RT Primer (100% over 22bp)
ATTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG12647510.9671524471319599Illumina RNA RT Primer (100% over 22bp)
CCTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG10625790.8125519411497053Illumina RNA RT Primer (100% over 22bp)
CCACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG10570790.8083461026413935Illumina RNA RT Primer (100% over 22bp)
CCTACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG8895850.6802637908029996Illumina RNA RT Primer (100% over 22bp)
CCAACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG8734930.6679582720255901Illumina RNA RT Primer (100% over 22bp)
CACCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG7964560.6090482390865335Illumina RNA RT Primer (100% over 22bp)
CTTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG7560950.5781842667167207Illumina RNA RT Primer (100% over 22bp)
CCTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG7557320.5779066813751722Illumina RNA RT Primer (100% over 22bp)
AGGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG7368110.5634378322086692Illumina RNA RT Primer (100% over 22bp)
TCCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG6681810.5109566146040447Illumina RNA RT Primer (100% over 22bp)
CAGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG6364210.4866698089633209Illumina RNA RT Primer (100% over 22bp)
CCCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG6225650.47607415471401776Illumina RNA RT Primer (100% over 22bp)
CTTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG6002260.45899156808907665Illumina RNA RT Primer (100% over 22bp)
AGTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG5864200.4484341487353036Illumina RNA RT Primer (100% over 22bp)
TACCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG5653840.4323479634878771Illumina RNA RT Primer (100% over 22bp)
TGGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG5406630.4134438664398817Illumina RNA RT Primer (100% over 22bp)
GCACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG5275060.40338273603008945Illumina RNA RT Primer (100% over 22bp)
TTTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG5244200.4010228782779713Illumina RNA RT Primer (100% over 22bp)
GGTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG5173720.39563328740404746Illumina RNA RT Primer (100% over 22bp)
AGCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG5125370.3919359730062861Illumina RNA RT Primer (100% over 22bp)
TGCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG4947340.37832205610383635Illumina RNA RT Primer (100% over 22bp)
TTCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG4774550.365108840906542Illumina RNA RT Primer (100% over 22bp)
TCCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG4709870.3601627748207674Illumina RNA RT Primer (100% over 22bp)
TAGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG4700860.3594737820033149Illumina RNA RT Primer (100% over 22bp)
AGCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG4611130.35261214765148197Illumina RNA RT Primer (100% over 22bp)
TCCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG4515800.3453222824697118Illumina RNA RT Primer (100% over 22bp)
GTGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG4486820.3431061879247868Illumina RNA RT Primer (100% over 22bp)
TGCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG4457250.3408449762031363Illumina RNA RT Primer (100% over 22bp)
CCCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG4286910.32781911199393954Illumina RNA RT Primer (100% over 22bp)
AGTTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG4238380.32410803303378743Illumina RNA RT Primer (100% over 22bp)
GGCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG4195720.3208458317471587Illumina RNA RT Primer (100% over 22bp)
TTTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG4177470.3194502580603098Illumina RNA RT Primer (100% over 22bp)
CCCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG4020660.3074590301241577Illumina RNA RT Primer (100% over 22bp)
CTAGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3987480.3049217624567798Illumina RNA RT Primer (100% over 22bp)
TGTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3978640.30424576950380755Illumina RNA RT Primer (100% over 22bp)
TTGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3941570.30141103434920546Illumina RNA RT Primer (100% over 22bp)
ATCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3906070.29869635676656786Illumina RNA RT Primer (100% over 22bp)
CATGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3858810.29508239444106216Illumina RNA RT Primer (100% over 22bp)
ACGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3797280.29037720819712726Illumina RNA RT Primer (100% over 22bp)
ACTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3765360.2879362924664852Illumina RNA RT Primer (100% over 22bp)
ATGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3653040.27934720553460207Illumina RNA RT Primer (100% over 22bp)
ACTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3633200.27783004487996743Illumina RNA RT Primer (100% over 22bp)
GCTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3456140.26429030367484607Illumina RNA RT Primer (100% over 22bp)
CTGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3424740.2618891522355553Illumina RNA RT Primer (100% over 22bp)
GCCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3288480.2514693785056907Illumina RNA RT Primer (100% over 22bp)
CATCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3222590.24643078397273321Illumina RNA RT Primer (100% over 22bp)
CTCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3157950.241487776678601Illumina RNA RT Primer (100% over 22bp)
TATCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3112300.23799693071670228Illumina RNA RT Primer (100% over 22bp)
ATTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3048830.2331433930781105Illumina RNA RT Primer (100% over 22bp)
GCAGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3047950.23307609966197754Illumina RNA RT Primer (100% over 22bp)
CTGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3046400.23295757148583418Illumina RNA RT Primer (100% over 22bp)
ACCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG3010810.23023601162200122Illumina RNA RT Primer (100% over 22bp)
GGCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2957690.22617393632088267Illumina RNA RT Primer (100% over 22bp)
ACACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2914890.22290102926350552Illumina RNA RT Primer (100% over 22bp)
CGCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2870580.21951265282162744Illumina RNA RT Primer (100% over 22bp)
TCAGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2836080.2168744450300501Illumina RNA RT Primer (100% over 22bp)
GCCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2794360.21368412534701803Illumina RNA RT Primer (100% over 22bp)
CCTTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2771580.21194214350666635Illumina RNA RT Primer (100% over 22bp)
TTGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2757790.210887625087946Illumina RNA RT Primer (100% over 22bp)
TGCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2689260.20564715030658956Illumina RNA RT Primer (100% over 22bp)
GCCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2685730.20537721194414704Illumina RNA RT Primer (100% over 22bp)
ACCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2655800.20308847109771483Illumina RNA RT Primer (100% over 22bp)
TGCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2410270.18431284329870062Illumina RNA RT Primer (100% over 22bp)
CACGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2408800.1842004327058421Illumina RNA RT Primer (100% over 22bp)
CGCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2390060.18276738882137372Illumina RNA RT Primer (100% over 22bp)
CACACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2358810.18037770785074206Illumina RNA RT Primer (100% over 22bp)
CCGACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2288880.17503017536190132Illumina RNA RT Primer (100% over 22bp)
AGCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2261300.17292113852446062Illumina RNA RT Primer (100% over 22bp)
AGTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2190080.16747496000515222Illumina RNA RT Primer (100% over 22bp)
CAACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2176290.16642044158643188Illumina RNA RT Primer (100% over 22bp)
CGCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2171290.1660380926311308Illumina RNA RT Primer (100% over 22bp)
TTCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2147350.16420740583314933Illumina RNA RT Primer (100% over 22bp)
GCGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2134900.16325535693444965Illumina RNA RT Primer (100% over 22bp)
AGACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2111750.1614850812714057Illumina RNA RT Primer (100% over 22bp)
GGCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2108710.16125261310658265Illumina RNA RT Primer (100% over 22bp)
GTTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2076150.1587627567096621Illumina RNA RT Primer (100% over 22bp)
CTCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2025950.1549239731984394Illumina RNA RT Primer (100% over 22bp)
ACCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2021990.15462115282584094Illumina RNA RT Primer (100% over 22bp)
AGGTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG2004240.15326381403452216Illumina RNA RT Primer (100% over 22bp)
CAGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1963760.15016831689240473Illumina RNA RT Primer (100% over 22bp)
GGTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1931140.14767387230802057Illumina RNA RT Primer (100% over 22bp)
CTACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1923400.1470819961252145Illumina RNA RT Primer (100% over 22bp)
ATCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1900390.145322426232919Illumina RNA RT Primer (100% over 22bp)
GCGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1870780.14305815571962607Illumina RNA RT Primer (100% over 22bp)
TAACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1858370.14210916561256884Illumina RNA RT Primer (100% over 22bp)
TCACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1847670.14129093884822455Illumina RNA RT Primer (100% over 22bp)
CTTACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1807250.13820002989357072Illumina RNA RT Primer (100% over 22bp)
CGCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1780160.13612846325374955Illumina RNA RT Primer (100% over 22bp)
AACCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1768920.13526894280223276Illumina RNA RT Primer (100% over 22bp)
CCCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1736440.132785203988597Illumina RNA RT Primer (100% over 22bp)
GACCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1702610.13019823095702998Illumina RNA RT Primer (100% over 22bp)
ACCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1666840.12746290653080614Illumina RNA RT Primer (100% over 22bp)
CAAGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1658730.1268427365253078Illumina RNA RT Primer (100% over 22bp)
GCTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1638440.1252911644646961Illumina RNA RT Primer (100% over 22bp)
AAGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1636470.12514051897630749Illumina RNA RT Primer (100% over 22bp)
TACGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1634010.12495240329029934Illumina RNA RT Primer (100% over 22bp)
TCTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1623340.12413647061968688Illumina RNA RT Primer (100% over 22bp)
ATGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1606460.12284566054659046Illumina RNA RT Primer (100% over 22bp)
CCATCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1574830.12042692105535592Illumina RNA RT Primer (100% over 22bp)
GGGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1568260.11992451452809032Illumina RNA RT Primer (100% over 22bp)
GGTTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1555210.11892658375475454Illumina RNA RT Primer (100% over 22bp)
TGACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1554530.11887458429683359Illumina RNA RT Primer (100% over 22bp)
ACTTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1548490.1184127067588299Illumina RNA RT Primer (100% over 22bp)
TCTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1521680.11636255166050558Illumina RNA RT Primer (100% over 22bp)
AATCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1497480.11451198271684841Illumina RNA RT Primer (100% over 22bp)
CCAGCACTAGCTGGAATTCTCGGGTGCCAAGGCCCGGAATGCCGAGACCG1482190.11334275961153774Illumina RNA RT Primer (100% over 22bp)
GTTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1472010.11256429713854477Illumina RNA RT Primer (100% over 22bp)
TATGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1469860.11239988708776531Illumina RNA RT Primer (100% over 22bp)
TTACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1446990.11065102296621823Illumina RNA RT Primer (100% over 22bp)
GTGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1414390.10815810777765526Illumina RNA RT Primer (100% over 22bp)
TACACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1402610.10725729363896595Illumina RNA RT Primer (100% over 22bp)
GCTACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1377700.10535243114365604Illumina RNA RT Primer (100% over 22bp)
TACTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1371500.10487831843908273Illumina RNA RT Primer (100% over 22bp)
ACAGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1362050.1041556789135637Illumina RNA RT Primer (100% over 22bp)
CGGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1357490.10380697666632913Illumina RNA RT Primer (100% over 22bp)
CGGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1356000.10369303667764941Illumina RNA RT Primer (100% over 22bp)
CTTTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1348050.1030851018387207Illumina RNA RT Primer (100% over 22bp)
TAGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1339210.10240910888574843Illumina RNA RT Primer (100% over 22bp)
TGTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1334730.10206652422179868Illumina RNA RT Primer (100% over 22bp)
ATCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1316110.1006426567122575Illumina RNA RT Primer (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)