FastQCFastQC Report
Fri 14 Jul 2023
SRR9693937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19030346
Total Bases970.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1400710.7360402170302106Illumina RNA RT Primer (100% over 22bp)
AGCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1354540.711778966078704Illumina RNA RT Primer (100% over 22bp)
CCAGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1318250.692709423149742Illumina RNA RT Primer (100% over 22bp)
ATTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1186290.6233675415045002Illumina RNA RT Primer (100% over 22bp)
CCGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1125980.591676052553117Illumina RNA RT Primer (100% over 22bp)
CCTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1000200.5255816158045682Illumina RNA RT Primer (100% over 22bp)
CCACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG915790.48122614270912367Illumina RNA RT Primer (100% over 22bp)
CCAACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG744470.3912015052169834Illumina RNA RT Primer (100% over 22bp)
CCTACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG734800.3861201472637439Illumina RNA RT Primer (100% over 22bp)
CACCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG725880.3814328967008797Illumina RNA RT Primer (100% over 22bp)
AGGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG691440.3633354853348436Illumina RNA RT Primer (100% over 22bp)
CCTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG613830.3225532525788023Illumina RNA RT Primer (100% over 22bp)
CTTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG576080.3027165139299096Illumina RNA RT Primer (100% over 22bp)
AGTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG549730.2888702076147223Illumina RNA RT Primer (100% over 22bp)
GCACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG542000.2848082741112537Illumina RNA RT Primer (100% over 22bp)
TCCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG532190.2796533494451441Illumina RNA RT Primer (100% over 22bp)
CAGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG480280.2523758632659648Illumina RNA RT Primer (100% over 22bp)
GGTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG464120.24388416269467725Illumina RNA RT Primer (100% over 22bp)
CCCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG458310.24083114411056952Illumina RNA RT Primer (100% over 22bp)
CTTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG455440.23932302649673315Illumina RNA RT Primer (100% over 22bp)
TACCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG453500.23830360204696224Illumina RNA RT Primer (100% over 22bp)
AGCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG450180.2365590199989007Illumina RNA RT Primer (100% over 22bp)
GGCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG435000.2285822864177036Illumina RNA RT Primer (100% over 22bp)
AGCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG423740.22266542079686832Illumina RNA RT Primer (100% over 22bp)
AGTTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG397140.20868774535155588Illumina RNA RT Primer (100% over 22bp)
TGCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG391240.2055874338806031Illumina RNA RT Primer (100% over 22bp)
TTTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG385550.20259747247895546Illumina RNA RT Primer (100% over 22bp)
CCCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG376340.19775783372514613Illumina RNA RT Primer (100% over 22bp)
TGGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG355530.18682266733353142Illumina RNA RT Primer (100% over 22bp)
TAGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG353730.18587680959663055Illumina RNA RT Primer (100% over 22bp)
GTGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG341430.1794134483944748Illumina RNA RT Primer (100% over 22bp)
TCCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG334380.17570883892494651Illumina RNA RT Primer (100% over 22bp)
TGCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG333170.17507301233514094Illumina RNA RT Primer (100% over 22bp)
ACTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG330960.1739117092248349Illumina RNA RT Primer (100% over 22bp)
TCCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG324380.17045407371994184Illumina RNA RT Primer (100% over 22bp)
TGTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG323370.16992334243423635Illumina RNA RT Primer (100% over 22bp)
TTCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG316510.16631857350360313Illumina RNA RT Primer (100% over 22bp)
ATCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG315610.16584564463515272Illumina RNA RT Primer (100% over 22bp)
ACGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG314880.1654620467751874Illumina RNA RT Primer (100% over 22bp)
CCCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG313170.1645634819251316Illumina RNA RT Primer (100% over 22bp)
GCTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG311830.16385934338766095Illumina RNA RT Primer (100% over 22bp)
ACTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG310270.1630396000156802Illumina RNA RT Primer (100% over 22bp)
ATGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG300690.15800553494928574Illumina RNA RT Primer (100% over 22bp)
CATGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG286180.15038087063682395Illumina RNA RT Primer (100% over 22bp)
CTAGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG283850.14915651034405786Illumina RNA RT Primer (100% over 22bp)
TTTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG283740.1490987079268028Illumina RNA RT Primer (100% over 22bp)
GCCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG276570.14533104127481444Illumina RNA RT Primer (100% over 22bp)
TATCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG270930.1423673536991918Illumina RNA RT Primer (100% over 22bp)
TTGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG267310.14046512869498012Illumina RNA RT Primer (100% over 22bp)
CTGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG261900.13762230071907258Illumina RNA RT Primer (100% over 22bp)
ACACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG258330.1357463495408859Illumina RNA RT Primer (100% over 22bp)
GGCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG256180.1346165750218099Illumina RNA RT Primer (100% over 22bp)
CTGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG254920.1339544746059793Illumina RNA RT Primer (100% over 22bp)
CGCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG250120.13143218730757708Illumina RNA RT Primer (100% over 22bp)
GCCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG248350.13050209386629125Illumina RNA RT Primer (100% over 22bp)
CATCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG247970.13030241278850105Illumina RNA RT Primer (100% over 22bp)
ATTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG244960.12872072846179464Illumina RNA RT Primer (100% over 22bp)
ACCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG242520.1274385657517735Illumina RNA RT Primer (100% over 22bp)
CGCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG230630.12119064992302295Illumina RNA RT Primer (100% over 22bp)
ACCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG223700.1175490976359547Illumina RNA RT Primer (100% over 22bp)
TTGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG219750.11547346537997785Illumina RNA RT Primer (100% over 22bp)
TGCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG219060.11511088658083253Illumina RNA RT Primer (100% over 22bp)
GCAGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG215000.11297745190760061Illumina RNA RT Primer (100% over 22bp)
CTCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG214580.11275675176899043Illumina RNA RT Primer (100% over 22bp)
AGTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG213310.11208939658795485Illumina RNA RT Primer (100% over 22bp)
CACGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG212250.11153239147622435Illumina RNA RT Primer (100% over 22bp)
GCCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG211410.11109099119900395Illumina RNA RT Primer (100% over 22bp)
TCAGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG209030.10984035708021284Illumina RNA RT Primer (100% over 22bp)
AGGTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG205950.10822188939707139Illumina RNA RT Primer (100% over 22bp)
CCTTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG199900.10504275644804355Illumina RNA RT Primer (100% over 22bp)
AGACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG198250.10417572018921778Illumina RNA RT Primer (100% over 22bp)
GCGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG197480.10377110326843242Illumina RNA RT Primer (100% over 22bp)
TGCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG196440.10322460768711195Illumina RNA RT Primer (100% over 22bp)
CGCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG195320.10263607398415142Illumina RNA RT Primer (100% over 22bp)
ACCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG192580.10119626831798013Illumina RNA RT Primer (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)