FastQCFastQC Report
Mon 24 Jul 2023
SRR9693938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39148096
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1389800.35501087971174894Illumina RNA RT Primer (100% over 22bp)
CCGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1230850.3144086496569335Illumina RNA RT Primer (100% over 22bp)
CACCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1220900.31186701902437347Illumina RNA RT Primer (100% over 22bp)
TCCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1208360.30866379810655414Illumina RNA RT Primer (100% over 22bp)
CTTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1131650.2890689754107071Illumina RNA RT Primer (100% over 22bp)
CCTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1108710.2832091757412672Illumina RNA RT Primer (100% over 22bp)
GGTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1086440.2775205210490952Illumina RNA RT Primer (100% over 22bp)
AGGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG1003640.2563700671419627Illumina RNA RT Primer (100% over 22bp)
GCACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG978460.24993808127986605Illumina RNA RT Primer (100% over 22bp)
ATTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG961320.24555983514498383Illumina RNA RT Primer (100% over 22bp)
CCACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG863020.22045005713687837Illumina RNA RT Primer (100% over 22bp)
TGCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG848930.21685090380896171Illumina RNA RT Primer (100% over 22bp)
GCTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG836490.21367322691760027Illumina RNA RT Primer (100% over 22bp)
TGTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG815830.20839583105140028Illumina RNA RT Primer (100% over 22bp)
CCTACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG808340.20648258346970438Illumina RNA RT Primer (100% over 22bp)
TCCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG807700.2063191017003739Illumina RNA RT Primer (100% over 22bp)
TTTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG777240.19853839124130074Illumina RNA RT Primer (100% over 22bp)
CCAACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG755470.19297745668141816Illumina RNA RT Primer (100% over 22bp)
AGTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG744500.19017527697898767Illumina RNA RT Primer (100% over 22bp)
GTGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG673880.17213608549442608Illumina RNA RT Primer (100% over 22bp)
ACTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG649450.16589567983076367Illumina RNA RT Primer (100% over 22bp)
AGTTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG627620.1603194188550064Illumina RNA RT Primer (100% over 22bp)
CCTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG601880.1537443864447456Illumina RNA RT Primer (100% over 22bp)
TACCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG598840.15296784804042576Illumina RNA RT Primer (100% over 22bp)
ATTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG593190.15152461054555502Illumina RNA RT Primer (100% over 22bp)
TGGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG591460.15108269888783352Illumina RNA RT Primer (100% over 22bp)
CATGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG581880.14863558115316772Illumina RNA RT Primer (100% over 22bp)
CTTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG566550.14471968189717324Illumina RNA RT Primer (100% over 22bp)
GCCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG551610.14090340434436455Illumina RNA RT Primer (100% over 22bp)
GGCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG540560.13808078942076774Illumina RNA RT Primer (100% over 22bp)
CCCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG532450.1360091688750329Illumina RNA RT Primer (100% over 22bp)
TGCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG526320.13444332005316426Illumina RNA RT Primer (100% over 22bp)
CCCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG491190.12546970355850767Illumina RNA RT Primer (100% over 22bp)
CGCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG485960.12413375097476007Illumina RNA RT Primer (100% over 22bp)
TGCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG463270.11833781137146492Illumina RNA RT Primer (100% over 22bp)
TGCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG457820.11694566192950993Illumina RNA RT Primer (100% over 22bp)
AGCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG457310.11681538739457469Illumina RNA RT Primer (100% over 22bp)
ACGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG456350.11657016474057895Illumina RNA RT Primer (100% over 22bp)
TCCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG447600.11433506242551361Illumina RNA RT Primer (100% over 22bp)
AGTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG446810.1141332646164963Illumina RNA RT Primer (100% over 22bp)
ATGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG426610.1089733712720026Illumina RNA RT Primer (100% over 22bp)
CTGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG421080.10756078660888133Illumina RNA RT Primer (100% over 22bp)
TTGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG419270.1070984397299935Illumina RNA RT Primer (100% over 22bp)
CAGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG414510.10588254407059797Illumina RNA RT Primer (100% over 22bp)
AGCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG404500.10332558702216323Illumina RNA RT Primer (100% over 22bp)
GCCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG391860.10009682207788599Illumina RNA RT Primer (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)