FastQCFastQC Report
Fri 14 Jul 2023
SRR9693939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5567824
Total Bases283.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG219130.3935648827980195Illumina RNA RT Primer (100% over 22bp)
CCAGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG180410.32402245473276453Illumina RNA RT Primer (100% over 22bp)
GGTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG173750.31206086974013547Illumina RNA RT Primer (100% over 22bp)
TCCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG172270.30940273974177346Illumina RNA RT Primer (100% over 22bp)
CCGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG172060.30902557264741126Illumina RNA RT Primer (100% over 22bp)
ATTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG143430.25760512544936764Illumina RNA RT Primer (100% over 22bp)
TGTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG134530.241620424783542Illumina RNA RT Primer (100% over 22bp)
CTTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG134090.24083016991916412Illumina RNA RT Primer (100% over 22bp)
CCTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG127200.2284554971565193Illumina RNA RT Primer (100% over 22bp)
CCACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG126190.2266415030360155Illumina RNA RT Primer (100% over 22bp)
TTTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG124270.22319311817327558Illumina RNA RT Primer (100% over 22bp)
CCTACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG122400.2198345349996695Illumina RNA RT Primer (100% over 22bp)
TGGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG121740.2186491527031027Illumina RNA RT Primer (100% over 22bp)
CACCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG121580.21836178729787437Illumina RNA RT Primer (100% over 22bp)
AGGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG119470.2145721560164258Illumina RNA RT Primer (100% over 22bp)
GTGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG115630.20767538629094598Illumina RNA RT Primer (100% over 22bp)
GCCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG114420.20550218541390677Illumina RNA RT Primer (100% over 22bp)
TGCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG112950.20286201575337154Illumina RNA RT Primer (100% over 22bp)
TACCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG111770.20074269588981258Illumina RNA RT Primer (100% over 22bp)
TGCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG110500.19846173298581274Illumina RNA RT Primer (100% over 22bp)
CTTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG110300.19810252622927735Illumina RNA RT Primer (100% over 22bp)
CCAACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG110210.19794088318883643Illumina RNA RT Primer (100% over 22bp)
CAGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG110200.19792292285100965Illumina RNA RT Primer (100% over 22bp)
GCTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG105460.1894097227211205Illumina RNA RT Primer (100% over 22bp)
ACTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG105110.18878111089718352Illumina RNA RT Primer (100% over 22bp)
GCACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG103660.18617686191230182Illumina RNA RT Primer (100% over 22bp)
CCCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG103230.1854045673857507Illumina RNA RT Primer (100% over 22bp)
GGCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG101240.18183046015822338Illumina RNA RT Primer (100% over 22bp)
TAGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG100870.1811659276586329Illumina RNA RT Primer (100% over 22bp)
TTGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG99900.17942377488943617Illumina RNA RT Primer (100% over 22bp)
GCAGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG99010.1778253048228536Illumina RNA RT Primer (100% over 22bp)
ATTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG98080.17615499340496396Illumina RNA RT Primer (100% over 22bp)
GCGGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG95780.17202411570480677Illumina RNA RT Primer (100% over 22bp)
CCTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG94260.16929414435513768Illumina RNA RT Primer (100% over 22bp)
AGTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG93780.1684320481394527Illumina RNA RT Primer (100% over 22bp)
TCCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG87650.15742236105164242Illumina RNA RT Primer (100% over 22bp)
CATGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG86330.15505159645850874Illumina RNA RT Primer (100% over 22bp)
GCCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG85610.15375845213498127Illumina RNA RT Primer (100% over 22bp)
ATGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG82260.14774173896301318Illumina RNA RT Primer (100% over 22bp)
AGCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG81500.14637675328817865Illumina RNA RT Primer (100% over 22bp)
TTCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG78640.14124009666972231Illumina RNA RT Primer (100% over 22bp)
GGGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG78110.1402881987649035Illumina RNA RT Primer (100% over 22bp)
TTTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG77100.1384742046443997Illumina RNA RT Primer (100% over 22bp)
ACGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG76930.13816887890134458Illumina RNA RT Primer (100% over 22bp)
CTGCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG73850.1326370948506993Illumina RNA RT Primer (100% over 22bp)
GCCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG71820.12899114627186492Illumina RNA RT Primer (100% over 22bp)
AGCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG71770.12890134458273106Illumina RNA RT Primer (100% over 22bp)
CCCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG70010.12574032512521946Illumina RNA RT Primer (100% over 22bp)
CTAGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG69400.12464474451778648Illumina RNA RT Primer (100% over 22bp)
AGTGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG69200.12428553776125106Illumina RNA RT Primer (100% over 22bp)
TCCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG69110.12412389472081015Illumina RNA RT Primer (100% over 22bp)
ATCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG67640.1214837250602749Illumina RNA RT Primer (100% over 22bp)
GGCACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG67360.12098083560112532Illumina RNA RT Primer (100% over 22bp)
CCCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG62380.11203658736339368Illumina RNA RT Primer (100% over 22bp)
CACACACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG62260.11182106330947243Illumina RNA RT Primer (100% over 22bp)
CTCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG61310.11011483121592924Illumina RNA RT Primer (100% over 22bp)
ACTCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG61150.10982746581070092Illumina RNA RT Primer (100% over 22bp)
CGCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG60480.10862412317630729Illumina RNA RT Primer (100% over 22bp)
TCCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG59820.10743874087974045Illumina RNA RT Primer (100% over 22bp)
ACCCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG59800.10740282020408691Illumina RNA RT Primer (100% over 22bp)
GGCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG59270.10645092229926809Illumina RNA RT Primer (100% over 22bp)
AGCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG58860.10571454844837049Illumina RNA RT Primer (100% over 22bp)
AGTTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG58080.10431364209788241Illumina RNA RT Primer (100% over 22bp)
ACACCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG58000.10416995939526826Illumina RNA RT Primer (100% over 22bp)
TGCTCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG56800.10201471885605579Illumina RNA RT Primer (100% over 22bp)
TATCCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG56630.1017093931130007Illumina RNA RT Primer (100% over 22bp)
TGCGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG56070.10070361419470156Illumina RNA RT Primer (100% over 22bp)
CACGCACTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACCG55820.1002546057490323Illumina RNA RT Primer (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)