FastQCFastQC Report
Fri 14 Jul 2023
SRR9693940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57934193
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC8981711.5503296990777105Illumina RNA RT Primer (100% over 22bp)
CCGCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC8331801.4381489701599883Illumina RNA RT Primer (100% over 22bp)
AGTTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC7489021.2926770206327032Illumina RNA RT Primer (100% over 22bp)
AGTCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC7291771.2586297698148656Illumina RNA RT Primer (100% over 22bp)
CCATTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC6877941.187198723903861Illumina RNA RT Primer (100% over 22bp)
AGACTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC6338821.0941414166242032Illumina RNA RT Primer (100% over 22bp)
TCCATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC6185691.0677097029728195Illumina RNA RT Primer (100% over 22bp)
TCCTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC5637180.9730315912055597Illumina RNA RT Primer (100% over 22bp)
AGGTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC5592550.9653280231244441Illumina RNA RT Primer (100% over 22bp)
CCTCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC5543480.9568580682568584Illumina RNA RT Primer (100% over 22bp)
CCTTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC4909840.8474856981264933Illumina RNA RT Primer (100% over 22bp)
AGGCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC4256350.7346870267097705Illumina RNA RT Primer (100% over 22bp)
CCTATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC3982680.6874489474635471Illumina RNA RT Primer (100% over 22bp)
CCCTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC3921840.6769473771732697Illumina RNA RT Primer (100% over 22bp)
TTCCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC3772100.6511008101899339Illumina RNA RT Primer (100% over 22bp)
TCCCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC3551640.6130472897067885Illumina RNA RT Primer (100% over 22bp)
CCCCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC3380000.5834205716820807Illumina RNA RT Primer (100% over 22bp)
AGATTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC3241620.5595348501704339Illumina RNA RT Primer (100% over 22bp)
AGCATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC3094050.5340628460985035Illumina RNA RT Primer (100% over 22bp)
CACCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2950860.5093468722348475Illumina RNA RT Primer (100% over 22bp)
CCAATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2860900.4938189093270014Illumina RNA RT Primer (100% over 22bp)
ACACTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2774430.47889335405086253Illumina RNA RT Primer (100% over 22bp)
ATCCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2761380.4766407982933326Illumina RNA RT Primer (100% over 22bp)
ACCCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2550130.4401770125632025Illumina RNA RT Primer (100% over 22bp)
CTCCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2418280.4174184319785036Illumina RNA RT Primer (100% over 22bp)
ATTCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2307770.398343341038685Illumina RNA RT Primer (100% over 22bp)
GCACTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2301290.3972248305935668Illumina RNA RT Primer (100% over 22bp)
CCCATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2299410.39690032447677315Illumina RNA RT Primer (100% over 22bp)
ACCTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2183320.3768620717647694Illumina RNA RT Primer (100% over 22bp)
AACCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2123760.3665814418093301Illumina RNA RT Primer (100% over 22bp)
AGCTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2059960.35556894699473934Illumina RNA RT Primer (100% over 22bp)
ACATTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2052170.3542243179256851Illumina RNA RT Primer (100% over 22bp)
AGCCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2052120.3542156874438555Illumina RNA RT Primer (100% over 22bp)
ATACTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2028960.3502180482603771Illumina RNA RT Primer (100% over 22bp)
CAATTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC2028520.35014210002027646Illumina RNA RT Primer (100% over 22bp)
ACTTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1963780.3389673521472889Illumina RNA RT Primer (100% over 22bp)
ATCTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1745090.30121935072091194Illumina RNA RT Primer (100% over 22bp)
ACTCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1716610.29630342827076234Illumina RNA RT Primer (100% over 22bp)
CTGCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1619640.2795654718104039Illumina RNA RT Primer (100% over 22bp)
ACCATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1603230.27673294767392376Illumina RNA RT Primer (100% over 22bp)
CACTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1601420.27642052423169167Illumina RNA RT Primer (100% over 22bp)
AACTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1587810.27407130707767Illumina RNA RT Primer (100% over 22bp)
TACCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1544210.26654552692224437Illumina RNA RT Primer (100% over 22bp)
ATTTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1528780.2638821602296247Illumina RNA RT Primer (100% over 22bp)
GCCATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1523040.2628913809155847Illumina RNA RT Primer (100% over 22bp)
CATTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1473170.25428333833872513Illumina RNA RT Primer (100% over 22bp)
AGTATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1436850.24801415633769164Illumina RNA RT Primer (100% over 22bp)
CACATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1343440.23189069018360195Illumina RNA RT Primer (100% over 22bp)
GCATTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1326270.22892698272331163Illumina RNA RT Primer (100% over 22bp)
CTTCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1280340.22099902211462583Illumina RNA RT Primer (100% over 22bp)
CATCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1261580.21776086533215366Illumina RNA RT Primer (100% over 22bp)
AATCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1260350.21754855547914512Illumina RNA RT Primer (100% over 22bp)
CTACTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1255420.2166975899707449Illumina RNA RT Primer (100% over 22bp)
CAACTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1253960.2164455799013201Illumina RNA RT Primer (100% over 22bp)
CTATTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1239050.21387197021972845Illumina RNA RT Primer (100% over 22bp)
CTTTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1217240.2101073540456497Illumina RNA RT Primer (100% over 22bp)
GCCCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1194370.20615977165678306Illumina RNA RT Primer (100% over 22bp)
ACGCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1134510.1958273588103661Illumina RNA RT Primer (100% over 22bp)
CTTATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1129480.19495913233830667Illumina RNA RT Primer (100% over 22bp)
CCAGTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1124000.1940132315297807Illumina RNA RT Primer (100% over 22bp)
CTCTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1120890.19347641555997855Illumina RNA RT Primer (100% over 22bp)
TCATTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1113450.1921921998637316Illumina RNA RT Primer (100% over 22bp)
CTCATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1111670.19188495471059724Illumina RNA RT Primer (100% over 22bp)
GCCTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1111650.1918815025178654Illumina RNA RT Primer (100% over 22bp)
ATGCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1092900.1886450718317592Illumina RNA RT Primer (100% over 22bp)
GAATTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1078320.186128423330243Illumina RNA RT Primer (100% over 22bp)
TACTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1074680.1855001242530469Illumina RNA RT Primer (100% over 22bp)
TGCTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1056950.18243975539626486Illumina RNA RT Primer (100% over 22bp)
TCACTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC1056800.18241386395077602Illumina RNA RT Primer (100% over 22bp)
TTCATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC993430.17147559127991996Illumina RNA RT Primer (100% over 22bp)
TTGCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC989420.1707834266371847Illumina RNA RT Primer (100% over 22bp)
GCTATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC965550.16666323461172577Illumina RNA RT Primer (100% over 22bp)
CGCTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC955140.1648663682947996Illumina RNA RT Primer (100% over 22bp)
GCAATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC944210.16297974496684542Illumina RNA RT Primer (100% over 22bp)
ATCATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC941040.1624325724188477Illumina RNA RT Primer (100% over 22bp)
AGGATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC932340.1609308685804944Illumina RNA RT Primer (100% over 22bp)
TATCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC917620.15839005472985532Illumina RNA RT Primer (100% over 22bp)
CGCATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC888850.15342407548509393Illumina RNA RT Primer (100% over 22bp)
TTACTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC879010.15172559666102536Illumina RNA RT Primer (100% over 22bp)
TTTCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC875960.15119913726941878Illumina RNA RT Primer (100% over 22bp)
ACTATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC866020.149483397481691Illumina RNA RT Primer (100% over 22bp)
TGCATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC865240.14934876196514899Illumina RNA RT Primer (100% over 22bp)
CAGCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC861590.14871873679158698Illumina RNA RT Primer (100% over 22bp)
TTCTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC860430.14851850961313987Illumina RNA RT Primer (100% over 22bp)
CTAGTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC849690.14666468211613823Illumina RNA RT Primer (100% over 22bp)
ATGTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC819520.14145704938014758Illumina RNA RT Primer (100% over 22bp)
TATTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC814490.14058882290808816Illumina RNA RT Primer (100% over 22bp)
CTAATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC803990.13877642172386864Illumina RNA RT Primer (100% over 22bp)
TGATTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC796320.1374525058112055Illumina RNA RT Primer (100% over 22bp)
GGCATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC793640.13698991198513802Illumina RNA RT Primer (100% over 22bp)
CATATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC778100.13430755823249318Illumina RNA RT Primer (100% over 22bp)
TTTTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC765490.13213095071506392Illumina RNA RT Primer (100% over 22bp)
CAGATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC759900.13116606284651278Illumina RNA RT Primer (100% over 22bp)
CGCCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC758860.13098654882445676Illumina RNA RT Primer (100% over 22bp)
TTATTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC754140.13017183133974095Illumina RNA RT Primer (100% over 22bp)
AATTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC749890.12943824038422352Illumina RNA RT Primer (100% over 22bp)
CAGTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC737210.12724955019223277Illumina RNA RT Primer (100% over 22bp)
GCTTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC726310.12536810515337635Illumina RNA RT Primer (100% over 22bp)
TGGCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC716430.1236627219438441Illumina RNA RT Primer (100% over 22bp)
GGTTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC707720.12215929200912491Illumina RNA RT Primer (100% over 22bp)
GCTCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC702110.12119095194784192Illumina RNA RT Primer (100% over 22bp)
TCTCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC701160.12102697279307922Illumina RNA RT Primer (100% over 22bp)
CGATTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC698010.12048325243781337Illumina RNA RT Primer (100% over 22bp)
TGCCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC693260.11966335666399978Illumina RNA RT Primer (100% over 22bp)
TAACTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC675860.11665994898729323Illumina RNA RT Primer (100% over 22bp)
GCGCTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC644520.11125036297648955Illumina RNA RT Primer (100% over 22bp)
TACATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC631270.10896328529164116Illumina RNA RT Primer (100% over 22bp)
TCAATCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC619720.10696964398899972Illumina RNA RT Primer (100% over 22bp)
CAGGTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC605700.10454965688397523Illumina RNA RT Primer (100% over 22bp)
TCTTTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC593870.10250768488308795Illumina RNA RT Primer (100% over 22bp)
GCAGTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC589310.10172058494022693Illumina RNA RT Primer (100% over 22bp)
TAATTCTCTAGCTGGAATTCTCGGGTGCCAAGGCCAGGAATGCCGAGACC586040.10115615142857Illumina RNA RT Primer (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)