FastQCFastQC Report
Tue 18 Jul 2023
SRR9693966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38693329
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA654981716.92750964901469No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA26156026.759826739126012No Hit
GGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCAC22800015.892491183686986No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGC15068193.894260429233163No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG11871203.068022397349166TruSeq Adapter, Index 9 (100% over 49bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT10124462.616590575600254No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC8486032.1931506591226615No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG6949191.795965914434501No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCT5812691.5022460331598761No Hit
AGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCC5340231.3801422979139377No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT3730740.9641817068776894No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACC3226470.8338569162658503No Hit
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCC2615440.6759408062304487No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT2403320.6211199868587166No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA2329210.6019668144862903No Hit
CCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCC2225270.5751043028631628No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2195440.5673949636124614No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1705310.4407245497020947No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGC1695900.4382926059424869No Hit
TTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGT1527950.39488719101941316No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT1343110.3471166825682019No Hit
GAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCC1299640.3358821878572402No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATACCCGTCGCCGCGC1263840.3266299469864689No Hit
TTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGG1235800.31938321977930617No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTCGGTCAC1213860.31371299171492845No Hit
GATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1202810.31085720228414565No Hit
GCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG1198270.3096838734139417No Hit
ATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA1103430.2851731883808705No Hit
ACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGAT1080130.27915147854039646No Hit
TGGCAGGGGAGATACCATGATCATGAAGGTGGTTTTCCCAGGGCGAGGCT1027390.2655212220173664No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1013640.2619676378840394No Hit
CTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTC1008990.2607658803407688No Hit
TCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCA1004310.2595563695230255No Hit
TACACCCGCAATCTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACC1003990.25947366792864995No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTCGGTCACG993130.2566669825695277No Hit
TCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGA991200.2561681885784498No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCC940800.24314268746429132No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTCGGTCA939930.24291784250458265No Hit
ATACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAG863280.22310822622679996No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG830700.21468817014943326TruSeq Adapter, Index 9 (100% over 50bp)
AGCTTGGAACAGACTCACGGCCAGCAGTGCGAGTTCAATGGCTGAGGTGA820430.21203396585494105No Hit
TTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTG811400.2097002302386543No Hit
TGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTC800130.20678758346173834No Hit
GTGCATTTATCAGACTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC792870.2049112910393417No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT784870.2028437511799515No Hit
GCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACC742290.19183927027834696No Hit
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGTGCACCA736260.19028086210933157No Hit
AGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA730570.18881032438434026No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA698670.18056600919502172No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGA677230.1750250023718559No Hit
CAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC644480.16656101107247712No Hit
GTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG637080.16464853670254118No Hit
GGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCG582260.15048071981606959No Hit
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCACACCA571660.14774122950237753No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTCGGTCAC566010.14628102947668317No Hit
TGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTC513950.13282651384170124No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAT510730.13199432904829667No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG472050.12199777382814489No Hit
GGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCG461450.11925828351445283No Hit
GGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTC460910.11911872457394401No Hit
TTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCCGAGGAAGCC457290.11818316278756993No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA453210.1171287174592809No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG446760.11546176344764753No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTG439190.11350535385569953No Hit
CCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCG424690.10975793786055472No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG411960.10646796505930001No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGT402050.10390680005848037No Hit
GTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGT399870.10334339544679652No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)