FastQCFastQC Report
Tue 25 Jul 2023
SRR9693969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53201588
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA556247610.455469863042435No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG48017209.025520065303313TruSeq Adapter, Index 10 (100% over 49bp)
GGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCAC21496024.040484656209886No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG20885093.9256516177674996No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT14202692.6695988849054655No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC12525172.3542849886360533No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCT7562941.4215628300418401No Hit
TCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCA7418911.3944903298751157No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGC6470741.2162682061294863No Hit
CGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGAT6040651.1354266342576091No Hit
AGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCC5272860.9911095134979805No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA5182020.9740348352007839No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC4867620.914938854832679No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT4744930.8918775131298712No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT4714400.8861389626189353No Hit
GTGCATTTATCAGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAG4402840.8275768009030106No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA4166850.7832191024072439No Hit
TACACCCGCAATCTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACC3745180.7039601900604922No Hit
TCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAG3481670.6544297136393749No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG3148220.5917530130867522No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACC2465540.4634335351042529No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCG2438900.45842616577535245TruSeq Adapter, Index 10 (100% over 50bp)
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCC2432890.45729650024732343No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGA2404620.4519827490863619No Hit
GAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATC2263520.4254609843600909No Hit
GTGCATTTATCAGACTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2081300.3912101270360576No Hit
GATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG2025350.3806935236594817No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1977630.3717238665883432No Hit
CGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATC1900930.357307003693198No Hit
TTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGG1893700.3559480217019086No Hit
TCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGA1803370.33896920520492735No Hit
CCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGA1695020.31860327176700065No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTCGGTCACG1666060.313159825229277No Hit
GTGCATTTATCAGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACA1658450.3117294167986113No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAG1446830.27195240863862935No Hit
TGGCAGGGGAGATACCATGATCATGAAGGTGGTTTTCCCAGGGCGAGGCT1444870.27158399858289944No Hit
GAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCC1420110.26693000216459706No Hit
CCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCC1341590.25217104421770264No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTCGGTCAC1158240.21770778721868228No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1156860.21744839646515812No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG1144050.21504057360092332No Hit
AGCTTGGAACAGACTCACGGCCAGCAGTGCGAGTTCAATGGCTGAGGTGA1119790.21048055933969492No Hit
AAGCGTGATGATTGGGTGTTCACGCCATTGCGTGACATGTGCCGCCCAAG1017130.19118414284926985No Hit
TGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTC952260.1789908977904945No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAGATCG942180.17709621750388352No Hit
CAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC921490.17320723584416314No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC911470.17132383341640103TruSeq Adapter, Index 10 (100% over 50bp)
TTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTG904840.17007763001360035No Hit
CTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTC824690.15501229023464488No Hit
ACCGCTCCTCTTATACACCCGCAATCTGTTACCGCTCCTCTTAGCTCGAC808770.15201989835341004No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCA782280.14704072367163176No Hit
TACCGCTCCTCTTATACACCCGCAATCTGTTACCGCTCCTCTTAGCTCGA765940.1439693867784548No Hit
GGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCG743740.13979657900437106No Hit
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT729110.13704666108838706TruSeq Adapter, Index 10 (100% over 48bp)
GTGCATTTATCAGATTTGGTGACTCTAGATAACCTCGGGCCGATCGCACA709880.13343210732732264No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGT687280.12918411382757972TruSeq Adapter, Index 10 (100% over 50bp)
GCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACC671370.1261936015894864No Hit
AGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA666870.12534776217582078No Hit
TTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAATAG658220.12372187085844129No Hit
TTACCGCTCCTCTTATACACCCGCAATCTGTTACCGCTCCTCTTAGCTCG653310.12279896607597501No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC650650.12229898100034158TruSeq Adapter, Index 10 (100% over 50bp)
GCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGT649060.12200011774084639No Hit
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGTGCACCA609650.114592444120277No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGAC605270.11376916042430915No Hit
GGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTC602410.11323158248584608No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAT601270.11301730316771746No Hit
CTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACCGCTCCTCTTAGC563420.10590285387721886No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGA560990.10544610059383941No Hit
GCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG545660.10256460765795186No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG539410.1013898306945274No Hit
TCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGA532870.10016054408000002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)