FastQCFastQC Report
Fri 14 Jul 2023
SRR9693970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41039411
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA434346910.583653357013336No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG30722777.4861625085213825TruSeq Adapter, Index 10 (100% over 49bp)
GGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCAC16420024.001036954453366No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG16268953.9642259972980605No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT10697862.6067284445188554No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC9521492.320084467099199No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCT5889961.4351960363173828No Hit
TCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCA5435231.3243927891655172No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGC4819041.1742468721103234No Hit
CGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGAT4610701.1234810363140935No Hit
AGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCC4258451.0376489077779405No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT3762810.9168771939733736No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA3669510.894142949566211No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT3668680.8939407049482265No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC3587110.8740646886964338No Hit
GTGCATTTATCAGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAG3375340.8224630709246777No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA3260550.7944923965892201No Hit
TACACCCGCAATCTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACC2751970.6705676160898119No Hit
TCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAG2577490.6280523860344877No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG2563560.6246580878073518No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACC2026170.49371322604995477No Hit
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCC1834960.4471214267670654No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGA1787010.43543753588471334No Hit
GAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATC1679760.409304119886126No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCG1649400.40190635289575666TruSeq Adapter, Index 10 (100% over 50bp)
GTGCATTTATCAGACTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1579790.38494460848865497No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1511830.36838491663537765No Hit
GATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1467670.35762452828574953No Hit
CGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATC1465400.3570714014389729No Hit
TTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGG1415630.34494403440634175No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTCGGTCACG1372270.33437858062826487No Hit
TCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGA1333680.32497542423306225No Hit
CCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGA1270940.3096876804591567No Hit
GTGCATTTATCAGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACA1262810.3077066578757673No Hit
TGGCAGGGGAGATACCATGATCATGAAGGTGGTTTTCCCAGGGCGAGGCT1139750.2777208474068987No Hit
GAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCC1137110.2770775633207796No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAG1084150.26417289468408794No Hit
CCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCC1063340.25910215914161144No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTCGGTCAC966880.23559792317682143No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC890080.21688420430790298No Hit
AGCTTGGAACAGACTCACGGCCAGCAGTGCGAGTTCAATGGCTGAGGTGA888480.2164943351648005No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG838250.20425488075352738No Hit
AAGCGTGATGATTGGGTGTTCACGCCATTGCGTGACATGTGCCGCCCAAG810550.19750527121356592No Hit
TGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTC737470.17969799810236067No Hit
CAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC728770.177578084636741No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAGATCG724300.17648888771819848No Hit
TTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTG684310.16674459582278117No Hit
CTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTC627960.15301389193914114No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC615950.15008743668372823TruSeq Adapter, Index 10 (100% over 50bp)
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCA607200.14795533980738662No Hit
ACCGCTCCTCTTATACACCCGCAATCTGTTACCGCTCCTCTTAGCTCGAC598730.1458914700310879No Hit
GGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCG596430.14533103313787812No Hit
TGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA587460.1431453292543599No Hit
TACCGCTCCTCTTATACACCCGCAATCTGTTACCGCTCCTCTTAGCTCGA556620.13563060152105985No Hit
GTGCATTTATCAGATTTGGTGACTCTAGATAACCTCGGGCCGATCGCACA545660.13295999789080792No Hit
GCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACC526670.12833273849861052No Hit
AGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA497600.12124930350486755No Hit
GCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGT496160.12089842127607532No Hit
TTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAATAG484240.11799389615996195No Hit
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT482580.11758940692399313TruSeq Adapter, Index 10 (100% over 48bp)
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGTGCACCA479610.11686571232710917No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGAC471510.11489199979015294No Hit
TTACCGCTCCTCTTATACACCCGCAATCTGTTACCGCTCCTCTTAGCTCG470880.11473848881505633No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGT468540.11416830519326898TruSeq Adapter, Index 10 (100% over 50bp)
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAT441060.10747230266048408No Hit
GGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTC439750.10715309729956894No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC432180.1053085289162654TruSeq Adapter, Index 10 (100% over 50bp)
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGA425130.10359066800447014No Hit
GCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG420600.10248685099306129No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG417920.10183382017836463No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)