FastQCFastQC Report
Wed 23 Aug 2023
SRR9693971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40196983
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA434232510.802614216096766No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG30545817.599030504354021TruSeq Adapter, Index 10 (100% over 49bp)
GGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCAC16499904.1047607975951825No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG16228954.037355241312513No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT10620992.6422356125582858No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC9432042.3464547078073No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCT5865561.4592040402634197No Hit
TCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCA5424261.3494196815716244No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGC4800051.1941319078598511No Hit
CGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGAT4557051.133679609735885No Hit
AGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCC4246401.0563976903440737No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT3749620.9328113007884199No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA3634310.9041250682918169No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT3619560.900455638673181No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC3552640.8838076230745975No Hit
GTGCATTTATCAGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAG3345420.8322564904933287No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA3244800.8072247611219977No Hit
TACACCCGCAATCTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACC2734420.6802550330705167No Hit
TCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAG2573330.6401798861372258No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG2553060.6351372191291073No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACC2028120.5045453286879764No Hit
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCC1811060.45054625119502134No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGA1770270.4403987234564345No Hit
GAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATC1696990.422168499561273No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCG1646140.40951829643533194TruSeq Adapter, Index 10 (100% over 50bp)
GTGCATTTATCAGACTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1572950.3913104622802164No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1498860.3728787307246417No Hit
GATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1475280.3670126188326124No Hit
CGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATC1455930.362198824722741No Hit
TTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGG1409990.3507701063037492No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTCGGTCACG1366820.3400304943283927No Hit
TCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGA1329740.33080592142947646No Hit
CCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGA1268200.31549631473585965No Hit
GTGCATTTATCAGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACA1243380.3093217219809756No Hit
GAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCC1136870.282824708511084No Hit
TGGCAGGGGAGATACCATGATCATGAAGGTGGTTTTCCCAGGGCGAGGCT1131940.2815982483063468No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAG1071530.2665697572377509No Hit
CCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCC1061720.26412927557274635No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTCGGTCAC953200.23713222457516278No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC880840.2191308735782484No Hit
AGCTTGGAACAGACTCACGGCCAGCAGTGCGAGTTCAATGGCTGAGGTGA876550.21806362930272655No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG829600.2063836482454417No Hit
AAGCGTGATGATTGGGTGTTCACGCCATTGCGTGACATGTGCCGCCCAAG797510.19840046204462658No Hit
TGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTC732020.1821081945378836No Hit
CAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC724910.18033940507425644No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAGATCG718910.1788467557378622No Hit
TTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTG673680.16759466749034374No Hit
CTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTC624530.15536738167638103No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC605810.15071031574683105TruSeq Adapter, Index 10 (100% over 50bp)
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCA605010.15051129583531184No Hit
GGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCG604430.15036700639946038No Hit
ACCGCTCCTCTTATACACCCGCAATCTGTTACCGCTCCTCTTAGCTCGAC587170.14607315180843297No Hit
TACCGCTCCTCTTATACACCCGCAATCTGTTACCGCTCCTCTTAGCTCGA549500.13670180172477123No Hit
GTGCATTTATCAGATTTGGTGACTCTAGATAACCTCGGGCCGATCGCACA537010.13359460335617723No Hit
GCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACC524010.13036052979398977No Hit
GCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGT497730.12382272570058304No Hit
AGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA487210.12120561386410518No Hit
TTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAATAG487080.12117327312848329No Hit
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT480620.11956618734296552TruSeq Adapter, Index 10 (100% over 48bp)
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGTGCACCA473670.1178372018616422No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGT465760.11586939248649582TruSeq Adapter, Index 10 (100% over 50bp)
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGAC465580.115824613006404No Hit
TTACCGCTCCTCTTATACACCCGCAATCTGTTACCGCTCCTCTTAGCTCG462870.1151504330561326No Hit
GGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTC441640.10986894215419102No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC434620.10812254243060977TruSeq Adapter, Index 10 (100% over 50bp)
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAT433780.10791357152351458No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGA424240.10554025907864777No Hit
GCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG421270.10480139765713262No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG415720.10342069702096797No Hit
CTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACCGCTCCTCTTAGC409390.10184595197107206No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)