FastQCFastQC Report
Tue 18 Jul 2023
SRR9693972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31368993
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA24186917.710451527723571No Hit
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA23248827.411401443457239No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGC22573637.196160233769697No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG21256006.776118060276912TruSeq Adapter, Index 4 (100% over 49bp)
ACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGAT15842725.050439457842972No Hit
ATACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAG15641034.9861434825147235No Hit
GGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCAC15042304.795276660618337No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCT8370502.6683993330611537No Hit
GCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG5870041.8712873569132422No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT4489081.4310564575662341No Hit
CCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCC4079411.300459342128069No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC3581941.141872804141338No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG3377111.0765758403529244No Hit
ATACTCTGGTTTCTCTTCAGATCGTACAAATCTTTCGCCTTTTACTAAAG2810180.8958464175117129No Hit
ACTCTGGTTTCTCTTCAGATCGTACAAATCTTTCGCCTTTTACTAAAGAT2667540.850374763384977No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGC2568590.8188308754444238No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG2113010.6735982886030164TruSeq Adapter, Index 4 (100% over 50bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATACCCGTCGCCGCGC1745940.5565814624651803No Hit
TACACCCGCAATCTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACC1670270.5324589157197364No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCC1657300.5283242595642136No Hit
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCC1552960.49506211436242153No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACC1535660.48954711424749914No Hit
TGGCAGGGGAGATACCATGATCATGAAGGTGGTTTTCCCAGGGCGAGGCT1533160.4887501489129728No Hit
TCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCA1428460.4553732407030089No Hit
AGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCC1364360.434939049525753No Hit
TACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGA1331760.42454662156352935No Hit
CTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTC1281490.4085212426168733No Hit
TTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGG1265280.4033537193878044No Hit
TCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGA1212540.3865409386906363No Hit
GAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCC1203170.3835539126168315No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA1179460.37599549338418353No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT1087090.34654921820410367No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1071650.3416271602980689No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGT908900.28974471702040294No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT901720.2874558325796432No Hit
TTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTG884910.282097037670288No Hit
TAACCCAAACTAATTATCTTCGGCGTAAAACGTGTCAACTATAAATAAAT852610.2718002455482074No Hit
TTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGT840720.2680098784172001No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC824310.26277859796136904No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG772370.24622084617124942No Hit
ATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA740070.23592405404916886No Hit
GTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGT733870.23394758001954352No Hit
AGCTTGGAACAGACTCACGGCCAGCAGTGCGAGTTCAATGGCTGAGGTGA677110.21585327906445706No Hit
TCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTT649020.2068985765657189No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC609920.19443403873372667Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTCGGTCA563900.17976350085576542No Hit
GTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG563610.1796710528769604No Hit
GTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCGGTGAGGCGGGG556710.17747142855366763No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGG550620.17553002099876142No Hit
TAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACG520820.16603019421120724No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT515690.16439482134475913No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC514030.16386563636263363No Hit
GGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCG512790.16347034155670856No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCGGT481980.15364854077400572No Hit
ACCCCGAGTGTTACAGCCCTCCGGAGCTTTCGCCGAATCCCGGGGCCGAG479100.15273043670863135No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTG454690.14494886718231598No Hit
CTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATT443300.14131789311821388No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT410820.13096371949204744TruSeq Adapter, Index 4 (100% over 50bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG398040.1268896327019487TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACTGACCAATCTCGTATG395780.12616917603953687TruSeq Adapter, Index 4 (97% over 49bp)
GGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTC389050.12402374535899192No Hit
AGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA383190.12215565861486213No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC373610.11910168745295711TruSeq Adapter, Index 4 (100% over 50bp)
GCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACC372360.11870320478569395No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT370660.11816126835821604No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCG370600.1181421411901874No Hit
GTGCATTTATCAGACTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC362830.11566517293047948No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTCGGTCAC361640.11528581743124493No Hit
ATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAA359880.11472475383573838No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTCACC348260.11102045896085985No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAT346340.11040838958394361No Hit
CGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGA340170.10844147913833255No Hit
GCCGCCGGTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCGGTGA337970.10774014964394936No Hit
AGATCGGAAAAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG337550.10760625946774893TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACATCTGAACTCCAGTCACTGACCAATCTCGTATG334710.106700906847727TruSeq Adapter, Index 4 (97% over 49bp)
TTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAATAA322680.10286590965798614No Hit
GAGCTTGGAACAGACTCACGGCCAGCAGTGCGAGTTCAATGGCTGAGGTG320200.10207532004613601No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)