FastQCFastQC Report
Tue 18 Jul 2023
SRR9693973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63243066
Total Bases7.9 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA46820567.403271688314415No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGC43538266.884274079944196No Hit
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA41071166.494175978122249No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG36011875.694200531011574TruSeq Adapter, Index 4 (100% over 49bp)
ACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGAT32206195.0924460240431735No Hit
ATACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAG31747695.019947957614831No Hit
GGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCAC27439114.338674851722084No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCT16495072.6082021387135153No Hit
GCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG11658621.8434621749679243No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT8876001.4034740187959895No Hit
CCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCC8341241.318917713445455No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC7209881.1400269556823825No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG6490211.0262326624076068No Hit
ATACTCTGGTTTCTCTTCAGATCGTACAAATCTTTCGCCTTTTACTAAAG5673080.8970279840638973No Hit
ACTCTGGTTTCTCTTCAGATCGTACAAATCTTTCGCCTTTTACTAAAGAT5397990.8535307254079049No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGC5316010.8405680395064971No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG3880350.6135613349295874TruSeq Adapter, Index 4 (100% over 50bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATACCCGTCGCCGCGC3506580.5544607846811221No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCC3362110.531617173651891No Hit
TACACCCGCAATCTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACC3344070.5287646870251357No Hit
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCC3169340.5011363617317351No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACC3041310.480892245167241No Hit
TGGCAGGGGAGATACCATGATCATGAAGGTGGTTTTCCCAGGGCGAGGCT3012070.47626881340635824No Hit
TCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCA2838370.44880335181725695No Hit
AGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCC2805770.443648636516136No Hit
TACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGA2728410.4314164654825558No Hit
CTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTC2669280.4220668238949706No Hit
TTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGG2581060.40811746856169184No Hit
TCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGA2457880.3886402344883153No Hit
GAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCC2417050.3821841907538132No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA2337460.3695994118944202No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2215490.3503135031435699No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT2202110.3481978561886927No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT1848600.292300819191783No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGT1834880.2901314114024769No Hit
TTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTG1796290.28402955669480034No Hit
TAACCCAAACTAATTATCTTCGGCGTAAAACGTGTCAACTATAAATAAAT1754820.277472316095491No Hit
TTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGT1713610.2709561867225096No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1629360.25763456819123853No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG1565970.24761133497227977No Hit
ATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA1493140.23609544799741367No Hit
GTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGT1481660.23428022923493302No Hit
AGCTTGGAACAGACTCACGGCCAGCAGTGCGAGTTCAATGGCTGAGGTGA1375430.21748313087793686No Hit
TCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTT1362480.21543547556660206No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTCGGTCA1174020.18563616128288277No Hit
GTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG1160750.18353790753914428No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGG1144980.18104435354225237No Hit
GTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCGGTGAGGCGGGG1089030.17219753387667827No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC1086110.17173582318099506No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT1064400.16830303578261055No Hit
GGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCG1048840.16584268700698349No Hit
TAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACG1046990.16555016481964996No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC995220.15736428717734843Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATT921960.1457804085589399No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTG915710.14479215792605626No Hit
ACCCCGAGTGTTACAGCCCTCCGGAGCTTTCGCCGAATCCCGGGGCCGAG900860.14244407442232482No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCGGT794140.1255694972157106No Hit
AGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA786790.12440731447143943No Hit
GGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTC779120.12319453329476468No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTCGGTCAC765340.12101563829938289No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT744650.11774413340428498TruSeq Adapter, Index 4 (100% over 50bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT741270.11720968746202153No Hit
GCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACC740390.11707054177291151No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCG733880.11604117991369994No Hit
ATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAA733630.1160016498883846No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTCACC723400.11438408125248072No Hit
GTGCATTTATCAGACTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC718810.11365830998769097No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAT715080.11306852200998604No Hit
CGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGA699790.11065086566169957No Hit
GCCGCCGGTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCGGTGA661720.10463123340667892No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC661410.1045822161752879TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACTGACCAATCTCGTATG658090.10405725743910012TruSeq Adapter, Index 4 (97% over 49bp)
TTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAATAA654350.10346588826038257No Hit
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG646160.1021708846310519TruSeq Adapter, Index 4 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)