FastQCFastQC Report
Fri 14 Jul 2023
SRR9693974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62021354
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA46720877.533029672328663No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGC43578127.026309035433183No Hit
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA41156986.635937035492646No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG35658485.7493875415876925TruSeq Adapter, Index 4 (100% over 49bp)
ACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGAT31934645.148974980455924No Hit
ATACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAG31303635.047234215492941No Hit
GGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCAC27681774.463264378265589No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCT16425772.64840557979434No Hit
GCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG11589071.8685612700425729No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT8832411.4240917732947267No Hit
CCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCC8345061.3455139982916207No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC7135941.1505617887671398No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG6482391.0451867916330881No Hit
ATACTCTGGTTTCTCTTCAGATCGTACAAATCTTTCGCCTTTTACTAAAG5600780.9030405882464287No Hit
ACTCTGGTTTCTCTTCAGATCGTACAAATCTTTCGCCTTTTACTAAAGAT5353240.8631285282807596No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGC5260830.8482288213185413No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG3877320.6251588767313916TruSeq Adapter, Index 4 (100% over 50bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATACCCGTCGCCGCGC3483130.5616017347831522No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCC3362370.5421310215188143No Hit
TACACCCGCAATCTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACC3313910.5343175835858082No Hit
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCC3137340.5058483566805072No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACC3047590.49137753426021624No Hit
TGGCAGGGGAGATACCATGATCATGAAGGTGGTTTTCCCAGGGCGAGGCT3006130.48469273985859773No Hit
TCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCA2820470.45475788871039485No Hit
AGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCC2786280.44924527123351743No Hit
TACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGA2720710.4386731060402196No Hit
CTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTC2642640.42608550596944406No Hit
TTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGG2541860.4098362638132666No Hit
TCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGA2446510.3944625265678656No Hit
GAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCC2424740.39095244518525024No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA2319280.37394862421094516No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT2213260.3568545117541291No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2192900.3535717714256932No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT1828160.29476299404879164No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGT1821570.29370045678138534No Hit
TTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTG1773840.2860047202452239No Hit
TAACCCAAACTAATTATCTTCGGCGTAAAACGTGTCAACTATAAATAAAT1741470.2807855500865073No Hit
TTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGT1689970.2724819583912986No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1626300.2622161392993774No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG1549260.249794611062506No Hit
ATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA1487390.23981901459294167No Hit
GTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGT1482280.23899510481502872No Hit
AGCTTGGAACAGACTCACGGCCAGCAGTGCGAGTTCAATGGCTGAGGTGA1367260.22044987924642856No Hit
TCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTT1346690.21713327961205103No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC1206870.19458943124653486Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTCGGTCA1160920.18718069263692633No Hit
GTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG1145280.18465898051822605No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGG1134660.18294666704632084No Hit
GTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCGGTGAGGCGGGG1082750.17457696908713086No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC1064970.1717102145174064No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT1049380.16919656413821602No Hit
TAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACG1038010.16736332457366218No Hit
GGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCG1038000.16736171222576016No Hit
CTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATT922120.1486778247375896No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTG915500.14761045042647733No Hit
ACCCCGAGTGTTACAGCCCTCCGGAGCTTTCGCCGAATCCCGGGGCCGAG900460.14518547918189595No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCGGT800270.12903136555193556No Hit
AGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA780370.12582279322699083No Hit
GGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTC778700.12555353112735979No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTCGGTCAC753380.12147106623954067No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT745810.12025051887774008TruSeq Adapter, Index 4 (100% over 50bp)
GCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACC743200.11982969607532269No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCG741880.11961686615226104No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT737450.11890259603168289No Hit
ATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAA732140.1180464392957303No Hit
GTGCATTTATCAGACTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC716560.11553440126444191No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTCACC715750.11540380108438135No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAT711340.11469275565960718No Hit
CGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGA698490.11262088860555994No Hit
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG662660.10684384607275745TruSeq Adapter, Index 4 (97% over 49bp)
GCCGCCGGTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCGGTGA661510.10665842606402949No Hit
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACTGACCAATCTCGTATG659550.10634240587524098TruSeq Adapter, Index 4 (97% over 49bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC654030.10545238983334675TruSeq Adapter, Index 4 (100% over 50bp)
TTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAATAA643810.10380457027752087No Hit
GAGCTTGGAACAGACTCACGGCCAGCAGTGCGAGTTCAATGGCTGAGGTG633080.10207452097869389No Hit
GGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCG623060.10045894838090766No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC622190.10031867411343519No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)