FastQCFastQC Report
Wed 23 Aug 2023
SRR9693975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34011019
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA395855211.639027927978281No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGC29927538.799362935876752No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG24418077.179458516076805TruSeq Adapter, Index 7 (100% over 49bp)
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA14805974.353286209978007No Hit
ATACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAG14183394.17023377041423No Hit
GGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCAC12041743.540540787678252No Hit
ACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGAT11122423.270240153639619No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT8920512.622829383618291No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCT6466381.9012602944945576No Hit
CCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCC5389591.5846599597618642No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC5234051.5389277222184963No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4875211.4334207393198068No Hit
TACACCCGCAATCTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACC4549101.337537108194259No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATACCCGTCGCCGCGC3115560.9160442972908279No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG3088890.9082027210063891No Hit
TTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGG2816150.8280110631204552No Hit
ATACTCTGGTTTCTCTTCAGATCGTACAAATCTTTCGCCTTTTACTAAAG2508580.7375786065098491No Hit
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCC2508470.7375462640504832No Hit
TTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTG2342370.6887091504079899No Hit
TCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGA2126740.62530910938011No Hit
TTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGT2079470.6114106725235136No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG2067940.6080205947372527TruSeq Adapter, Index 7 (100% over 50bp)
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA1925840.5662400176836807No Hit
ACTCTGGTTTCTCTTCAGATCGTACAAATCTTTCGCCTTTTACTAAAGAT1801250.5296077721164426No Hit
CTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTC1571750.462129640984882No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT1392940.40955550317383904No Hit
TACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGA1240950.3648670450009157No Hit
TGGCAGGGGAGATACCATGATCATGAAGGTGGTTTTCCCAGGGCGAGGCT1206810.3548291217031751No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACC1161230.3414275826313819No Hit
GTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGT1133100.3331567337044503No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGC1131870.33279508620426806No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG1101890.32398029591527383No Hit
AGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCC1091500.3209254036169866No Hit
TAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACG1088140.3199374884945376No Hit
GCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG1080030.3175529671721979No Hit
TAACCCAAACTAATTATCTTCGGCGTAAAACGTGTCAACTATAAATAAAT989940.2910644929515343No Hit
TCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTT954970.28078253109676016No Hit
GTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG947620.2786214667664029No Hit
TCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCA913260.2685188585499306No Hit
TTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCCGAGGAAGCC877190.25791347210149745No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC842600.24774323874271453No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT834100.24524404870080485No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT751780.22104012820080457No Hit
GAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCC741330.21796759456104506No Hit
ATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA731180.21498326762864703No Hit
GGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCG699780.2057509655914749No Hit
ACCCCGAGTGTTACAGCCCTCCGGAGCTTTCGCCGAATCCCGGGGCCGAG660910.19432231654100102No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCC648730.19074112422212344No Hit
AGCTTGGAACAGACTCACGGCCAGCAGTGCGAGTTCAATGGCTGAGGTGA643040.1890681370058333No Hit
AGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA626320.1841520831822181No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT615280.18090607635131425No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC560500.16479953158710123No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAT531340.15622583963156175No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC522540.1536384428822906No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCG519600.1527740171501477No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGA512800.15077466511662002No Hit
ACACCCGCAATCTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACCG504780.14841660580648877No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT458580.13483277287281512TruSeq Adapter, Index 7 (100% over 50bp)
GCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACC434580.12777623628389376No Hit
GATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG404660.11897908733637177No Hit
CTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATT386080.11351615192711514No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATACCCGTCGCCGCGCT385930.11347204857343439No Hit
TCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGA377300.1109346356250014No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT372550.10953802942511072No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC365180.10737108464759612TruSeq Adapter, Index 7 (100% over 50bp)
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG364090.10705060027751594No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG346250.10180524141308439No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCATACCCCGAAAACGTTGGT342760.10077910338411207No Hit
ATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAA341690.10046449946118934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)