FastQCFastQC Report
Tue 18 Jul 2023
SRR9693976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59280586
Total Bases7.4 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA653475611.023433540282479No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGC49582888.364100854198709No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG38355206.470111479667222TruSeq Adapter, Index 7 (100% over 49bp)
ATACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAG24671314.161785782616926No Hit
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA22356243.7712582665765146No Hit
ACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGAT19424213.2766562057939175No Hit
GGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCAC18748473.1626661045489666No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT15215302.566658163601824No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCT10990581.8539931437249961No Hit
CCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCC9470541.5975786744078408No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC9057141.5278425216646814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8739021.4741790845319915No Hit
TACACCCGCAATCTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACC7825461.3200712961913028No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATACCCGTCGCCGCGC5380640.907656344692679No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG5115260.8628895807473967No Hit
TTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGG4936600.8327515520848596No Hit
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCC4397140.7417504273658834No Hit
ATACTCTGGTTTCTCTTCAGATCGTACAAATCTTTCGCCTTTTACTAAAG4351110.73398565931855No Hit
TTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTG4118030.6946675594603603No Hit
TCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGA3699080.6239951811542483No Hit
TTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGT3674950.619924708571538No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG3413640.5758445100390877TruSeq Adapter, Index 7 (100% over 50bp)
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA3307580.5579533238757121No Hit
ACTCTGGTTTCTCTTCAGATCGTACAAATCTTTCGCCTTTTACTAAAGAT3169410.534645524590462No Hit
CTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTC2821310.4759247825249231No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT2470630.4167688220895792No Hit
TACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGA2176200.3671016342517262No Hit
TGGCAGGGGAGATACCATGATCATGAAGGTGGTTTTCCCAGGGCGAGGCT2044140.344824526532177No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGC2017380.3403104011151307No Hit
GTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGT1997060.33688263472969043No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACC1987840.33532731947015504No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG1935190.3264458283189036No Hit
AGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCC1916340.32326603519067776No Hit
TAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACG1899530.32043036821532095No Hit
GCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG1832820.30917710563792333No Hit
TAACCCAAACTAATTATCTTCGGCGTAAAACGTGTCAACTATAAATAAAT1742930.2940136253039064No Hit
TCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTT1710370.28852110200125214No Hit
GTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG1667810.2813416857923773No Hit
TCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCA1574780.26564852108580705No Hit
TTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCCGAGGAAGCC1525260.2572950274142027No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1454210.24530965331550536No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1434180.2419308068243455No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT1308620.22075017949383968No Hit
ATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA1287030.21710817770930943No Hit
GAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCC1275170.2151075227225318No Hit
GGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCG1211610.20438563141059368No Hit
AGCTTGGAACAGACTCACGGCCAGCAGTGCGAGTTCAATGGCTGAGGTGA1129170.19047888629171109No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCC1127950.19027308535715218No Hit
AGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA1106020.1865737292138104No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT1105350.18646070738909362No Hit
ACCCCGAGTGTTACAGCCCTCCGGAGCTTTCGCCGAATCCCGGGGCCGAG1083540.1827815939606265No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC997200.16821696060831787No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAT947920.15990395236646276No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC919000.1550254580816728No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCG889790.15009804390260242No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGA879290.14832680635107082No Hit
ACACCCGCAATCTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACCG874820.14757276522199023No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT768110.12957193101971023TruSeq Adapter, Index 7 (100% over 50bp)
GCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACC743620.125440730292376No Hit
CTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATT682020.11504946999005712No Hit
GATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG680020.114712091408813No Hit
TCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGA661980.11166893660599103No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT659740.11129107259499763No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATACCCGTCGCCGCGCT654640.11043075721282511No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG647630.10924824528556448No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC614570.10367137733759921TruSeq Adapter, Index 7 (100% over 50bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG613270.10345208125979051No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCATACCCCGAAAACGTTGGT606270.10227125622543609No Hit
ATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAA601390.10144805248720044No Hit
AGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCCGAGGA598540.10096728800892758No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)