FastQCFastQC Report
Wed 23 Aug 2023
SRR9693977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58132240
Total Bases7.3 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA651314711.20401863062562No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGC49557428.524945882009707No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG37985486.534322434504503TruSeq Adapter, Index 7 (100% over 49bp)
ATACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAG24309414.181743211684257No Hit
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA22367453.8476841766290097No Hit
ACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGAT19197683.302415320655113No Hit
GGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCAC18887583.24907142749015No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT15104672.5983292575686057No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCT10934101.880901200435421No Hit
CCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCC9458171.6270093841214446No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC8991221.5466839055229937No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8640051.486275085907579No Hit
TACACCCGCAATCTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACC7806391.3428675722800292No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATACCCGTCGCCGCGC5350850.9204616921694398No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG5108760.8788169869249833No Hit
TTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGG4869250.8376160973669688No Hit
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCC4357450.7495754507309541No Hit
ATACTCTGGTTTCTCTTCAGATCGTACAAATCTTTCGCCTTTTACTAAAG4293950.738652080153801No Hit
TTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTG4068340.6998422906118876No Hit
TCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGA3677330.6325801310942086No Hit
TTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGT3619490.6226304026818853No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG3416400.5876945392092237TruSeq Adapter, Index 7 (100% over 50bp)
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA3277520.5638041816382785No Hit
ACTCTGGTTTCTCTTCAGATCGTACAAATCTTTCGCCTTTTACTAAAGAT3121000.5368793633274754No Hit
CTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTC2781230.4784315897684314No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT2441910.42006122592213896No Hit
TACTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGA2158680.37133955271635843No Hit
TGGCAGGGGAGATACCATGATCATGAAGGTGGTTTTCCCAGGGCGAGGCT2024730.3482972615540017No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGC1995840.3433275579953568No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACC1985320.3415178909328111No Hit
GTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGT1981280.34082292373388673No Hit
AGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCC1909980.32855778480237474No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG1907120.3280658030724431No Hit
TAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACG1885740.3243879816088284No Hit
GCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG1827820.3144244914697937No Hit
TAACCCAAACTAATTATCTTCGGCGTAAAACGTGTCAACTATAAATAAAT1731530.2978605331568162No Hit
TCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTT1685330.28991313598099777No Hit
GTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG1656580.2849675154440978No Hit
TCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCA1563300.2689213420986358No Hit
TTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCCGAGGAAGCC1510470.2598334418216122No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1440880.24786245979855587No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1425120.2451513996364152No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT1313700.22598475475914914No Hit
GAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCC1280360.22024955515218403No Hit
ATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA1265560.21770363571057988No Hit
GGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCG1209370.2080377429116786No Hit
AGCTTGGAACAGACTCACGGCCAGCAGTGCGAGTTCAATGGCTGAGGTGA1119360.19255408014554404No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCC1116730.19210166337990758No Hit
AGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA1101140.18941984688702862No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT1085380.18670878672488794No Hit
ACCCCGAGTGTTACAGCCCTCCGGAGCTTTCGCCGAATCCCGGGGCCGAG1083610.18640430852139878No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC992980.17081399237325107No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAT926200.15932639100093168No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC907190.15605626069114142No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCG892470.15352410297624863No Hit
ACACCCGCAATCTGTTACCGCTCCTCTTATACACCCGCAATCTGTTACCG876590.15079240022404092No Hit
ATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGA871080.14984456129679502No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT766150.1317943364989892TruSeq Adapter, Index 7 (100% over 50bp)
GCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACC745890.12830917920933377No Hit
GATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG682570.11741677251728129No Hit
CTCTGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATT678360.11669256164909524No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATACCCGTCGCCGCGCT654790.11263801291675671No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT652760.11228880910145557No Hit
TCTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGA652710.11228020802226096No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG641610.11037076844105784No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC612560.10537354142899018TruSeq Adapter, Index 7 (100% over 50bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG609660.10487467883570287No Hit
ATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAA598620.10297556054953327No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCATACCCCGAAAACGTTGGT596760.10265560040349382No Hit
AGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCCGAGGA594750.10230983701987055No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)