FastQCFastQC Report
Fri 14 Jul 2023
SRR9693978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36275339
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC17476674.817782681507126No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG8070252.2247207669099938No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC8026282.2125995845276596No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA7399802.039898234996508No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA7276932.006026738992019No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA7004041.9307993234742755No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5353761.4758676686660321No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG4991761.3760753552158396No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4119671.135666850694352No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC3635321.002146389314239No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC3515980.9692480062005762No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT3226500.8894472357653225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG2804280.7730541126025039No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG2334880.6436549083662595No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2272370.6264228157867802Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC1852550.5106912991219738No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1782440.4913641192987886No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGGAAGAGCACACGT1742490.48035112780062506No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA1671340.46073725182830133No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1630120.4493741602249396No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC1614060.44494691007574044No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1367910.37709089362335113No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1292080.3561868849799033Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1246140.3435226339304506No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT1180210.3253477520913037No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1136510.3133010004399959No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGGAAGAGCACACG1119450.3085980809166249No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1110080.3060150588806351TruSeq Adapter, Index 2 (100% over 49bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGAGATCGGAAGAGCACACGTC1057030.29139079858082095No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1032250.2845597114888437No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT982380.2708120797988959No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG978610.2697728062582682No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG974190.2685543476244288No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT831220.22914189719908615No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT761730.20998563238788753No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC760720.20970720631997403No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCAGATCGGAAGAGCACAC694940.19157367488695282No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA661170.18226432012117103Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT650050.1791988766803806Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA631340.17404110268962614No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT608300.1676896803087078No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG607880.16757389917155563No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT606600.16722104237261573No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC575180.15855951063613766No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAA526980.14527224680105677Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACACCCGCAATCTGTTACCGCAGATCGGAAGAGCACACGTCTGAACTC521280.1437009313682775Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC488140.13456524830822394No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT486510.1341159072283239No Hit
TATACACCCGCAATCTGTTACCGCAGATCGGAAGAGCACACGTCTGAACT484800.1336445125984901Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGAGATCGGAAGAGCACACGTCTG456190.12575761180343484Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCAGATCGGAAGAGCACACG452560.12475693197519118No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC440250.1213634419791363No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCAGATCGGAAGAGCACACGT435480.1200484990643368No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACA434670.11982520687125764No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG428690.11817670401371025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT411800.11352064828394849Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGTC410790.11324222221603497No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGT404560.11152480201494465No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA399260.11006375433183409No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)