FastQCFastQC Report
Wed 23 Aug 2023
SRR9693979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51331766
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC16796313.2721083471003123No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC9304461.8126124863890325No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA8759001.7063508003991135No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA8603161.6759914318942388No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG7725381.5049901069057317TruSeq Adapter, Index 5 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA6842361.3329679715285851No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG6236471.2149338481750267No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG5310181.0344822346458915No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5233921.0196259368906186No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3487810.6794642522137266No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT3222080.6276970872188579Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3143550.6123985681692697Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC3125320.608847161034748No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGGAAGAGCACACGT2497100.4864629048609004No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2473400.4818458807748793Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC2462780.47977698643759886No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC2338710.4556067679417069No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCT2139310.4167614260534111Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG2103070.409701470235799No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1957540.3813506046139149No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1804150.35146852340907186No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTG1787610.3482463471060006Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG1670180.325369674598766No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1666060.32456705269014124No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1659610.32331052081862915No Hit
TTACCGCTCCTCTTAGCTCGACGCAGATCGGAAGAGCACACGTCTGAACT1620900.315769381478128Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC1383760.26957186705791497No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1376180.2680951985949597Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1370900.2670665957606056Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAA1348270.2626580195974555Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1322850.25770592034569784Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1320320.2572130481542365No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC1309450.25509545103123865No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1298230.2529096700082362No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGAGATCGGAAGAGCACACGTC1292070.2517096333681565No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC1232560.24011642225595745Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1224420.23853065955299493No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC1107510.21575528884005277Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCAGATCGGAAGAGCACACGTC1098810.21406043189708296No Hit
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1039500.202506183013458Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGAGATCGGAAGAGCACACGTCTG1037820.20217890029343624Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGAGCCGAAGGGAAACCGAGTAGATCGGAAGAGCACACGTCTGAACT1001200.19504491624153356Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGGAAGAGCACACG995730.19397929929003416No Hit
GAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCT970530.1890700584897079Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCGCTCCTCTTAGCTCGACGCAGATCGGAAGAGCACACGTCTGAACTC940350.18319065819788863Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC920290.17928274667191463No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT908270.17694111673461616No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACG884670.17234357376288204No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA871720.16982076946271438No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCAGATCGGAAGAGCACACGTCTGA870610.16960452909412857Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG866670.1688369731912204No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC851550.16589142871102466Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA835920.16284653054796516Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCTGTTACCGCTCCTCTTAGCTCGACGCAGATCGGAAGAGCACACGTC786650.15324818553875585No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT772220.15043706074714047No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACACGTC767120.14944352391850302No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT763760.14878895847845952No Hit
CAATCTGTTACCGCTCCTCTTAGCTCGACGCAGATCGGAAGAGCACACGT750910.14628563529257887No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACACGT739150.14399465625242663No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCAGATCGGAAGAGCACACG707380.13780550624344387No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACGTC669350.13039683848009437No Hit
CTGTTACCGCTCCTCTTAGCTCGACGCAGATCGGAAGAGCACACGTCTGA648610.1263564553769687Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACGTACGCAGTTTTATCCGGTAAAAGATCGGAAGAGCACACGTCTGA643990.12545642789690892Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCTCCTCTTAGCTCGACGCAGATCGGAAGAGCACACGTCTGAACTCC638770.12443951373112704Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGTTACCGCTCCTCTTAGCTCGACGCAGATCGGAAGAGCACACGTCTG636420.12398170754538233Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTG630290.12278751523958867Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG606000.1180555525792742No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT603790.11762501995353132Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT602050.11728604856493735No Hit
TTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAACT583300.11363333963612318Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC560040.10910203245296489Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCAGATCGGAAGAGCACAC557800.1086656554929359No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA556280.10836954255577336No Hit
ATCTGTTACCGCTCCTCTTAGCTCGACGCAGATCGGAAGAGCACACGTCT554840.10808901451004042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTACCGCTCCTCTTAGCTCGACGCAGATCGGAAGAGCACACGTCTGAAC540490.10529347460985464Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC520060.10131348296101872No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)