FastQCFastQC Report
Tue 18 Jul 2023
SRR9693980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33929124
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC17265275.088628282887586No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA14639174.314632467375226No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA10775073.175758383859247No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA5444231.6045890250511625No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC5175431.5253650521599085No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3730561.099515566626477No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG3231840.952526802637168TruSeq Adapter, Index 11 (100% over 49bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC3137400.9246923085901069No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG2744840.808992298180171No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2659370.7838015505499051No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG2587160.7625189497966408No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC2506220.7386633383166628No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2218710.6539249289194735No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2150860.6339273598693559No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG2135760.6294769060350629No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2015940.5941621127618857No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1753060.5166829535593079Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGAGATCGGAAGAGCACACGTC1647850.48567419541984047No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1532510.4516798016948508No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1500280.44218058798099236No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT1350090.3979147825920882No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1296300.3820611460525771Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT1237130.364621851127073Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGGAAGAGCACACG1209150.3563752485917408No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1103200.32514838874118884No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1062270.31308500626187696No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1031030.30387757726960474Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGAGATCGGAAGAGCACACGT881340.2597591379017035No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC841550.24803174995027868Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC827560.2439084486826126Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG827030.24375224070034934No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA820540.2418394297477294Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG816930.2407754470760872No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT711960.20983742462670124No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA684900.2018619755700147No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGGAAGAGCACACGT631880.18623528270284845No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA622920.1835944836064733No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT620730.18294902043447983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC599410.17666533329890863No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC598980.176538598520846No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT587390.17312265415399467No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC577490.17020480693813372No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC568710.16761705960931972No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCAC559240.16482594717152144No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC556140.16391227784130236No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC512480.1510442768873137No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA498970.14706244699981055Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT491630.14489911381148537No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC477660.1407817071846594No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC443240.13063703029880758Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAA434730.12812886062133522Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC431130.12706782527011307No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC418740.12341609526965683No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT416470.12274705353430286Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT415880.12257316162951923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT412340.12152981020081746No Hit
GAGAGAGACGGGGAGGGCGGCGCCGCCGCAGATCGGAAGAGCACACGTCT411830.12137949685939431Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCAGATCGGAAGAGCACAC392280.11561748543817402No Hit
CCGACGTACGCAGTTTTATCCGGTAAAAGATCGGAAGAGCACACGTCTGA390480.11508696776256293Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC386300.1138549878269772No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG385290.11355730846455098No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA373960.11021799442862126Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC355650.10482145073948859No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC351440.10358062884264269Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCAGATCGGAAGAGCACACGT350430.10328294948021646No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)