FastQCFastQC Report
Wed 23 Aug 2023
SRR9693981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9693981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44125723
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC16461263.7305360413018045No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA12619822.859969002660874No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA12591272.8534988537184987No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT7150991.6205944092972708No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT4822491.092897673314044No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4798311.0874178764164386No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC4461551.0110995801700517No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC4024970.9121595582694476No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA3762170.8526024604741321No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG3662940.8301144436772175No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG3657220.8288181476369237No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT3575100.810207687701797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC3509710.7953886670593477No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG3170670.7185536653982985No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC2778340.6296418077954213No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2721520.6167649649615939No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG2417670.5479049034505339No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG2214650.5018954590273796No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC2146710.48649854417116295No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT2023820.458648575571215No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1802230.4084307015207434TruSeq Adapter, Index 12 (100% over 49bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1684810.38182037266562185No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1668880.37821023351844No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC1588790.36005982270250847No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1562520.35410637917479565Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAA1519600.3443796263689549Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1450840.32879687886360526No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGAGATCGGAAGAGCACACGTC1366560.3096969085356403No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1341470.3040108827225335Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1299690.2945424826240241Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1269170.2876258820733657Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1134680.2571470613637311No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT1095670.2483064130190003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGGAAGAGCACACGT1082260.24526736933012971No Hit
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1072220.2429920525041595Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGGAAGAGCACACG1046830.23723803913649186No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG975990.22118391125285358No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC967990.21937090979789725No Hit
CCGACGTACGCAGTTTTATCCGGTAAAAGATCGGAAGAGCACACGTCTGA947470.21472056106593426Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC818070.18539526253201563No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA783470.1775540312393295No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA754510.17099096597238758Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG744230.1686612591027687No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT736440.16689584893600498No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC734210.1663904747804359Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGAGATCGGAAGAGCACACGT732380.16597575069761464No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC728920.16519162756834602Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC726650.16467718840550216No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC720970.16338995737248316No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT697300.15802573931763111No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG645660.14632281492588803No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA626130.14189682512397586No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA621570.14086341429465077Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC580440.13154232056435652No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACA579390.13130436412339352No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC572530.12974971537576846No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTCT569280.12901318353469246Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG563780.12776674503441Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCCCGGCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGA562780.12754011985254043Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA540900.12258156087323487Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC533040.12080028694374029No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCAC526160.11924110569247783No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT510530.11569895409985691Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCAGATCGGAAGAGCACACGT508580.1152570349952113No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT500770.11348709232481018No Hit
GAGAGAGACGGGGAGGGCGGCGCCGCCGCAGATCGGAAGAGCACACGTCT490740.11121404175065867Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT471240.10679485070420262Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGT458030.10380113205170599No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAGC456800.10352238307800646No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT454790.10306686646244867Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC453580.10279264999238653No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)