FastQCFastQC Report
Tue 27 Jun 2023
SRR970561.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR970561.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110397778
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT8807330.7977814553477698No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7632010.6913191676738276Illumina Single End Adapter 1 (95% over 22bp)
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATTC7007180.6347211082454939No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG5345420.48419633953139896No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATT4809810.43567996450073476No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA4579560.4148235664670714No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATTCGTATGCCG4339270.39305772983945386No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3926630.35568016595406476No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT3632280.3290174916382828No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC3350910.3035305656242465No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATTC3128710.28340334893334534No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT2850140.2581700512124438Illumina Single End Adapter 1 (95% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA2803020.2539018493651204No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATTC2492130.22574095648917863No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATT2466110.22338402499369145No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATT2465960.2233704377637021No Hit
AGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTCTGTAGGCACCATC2426720.21981601839848622No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACGCTGTAGGCACCATCAATT2422320.21941745965213177No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATTCGTATGCC2392090.21667917990160998No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA2328000.21087380943482395No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC2301170.20844350689739424Illumina Single End Adapter 1 (95% over 22bp)
AGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCAATTCGTATG2233470.20231113709553106No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG2094840.1897538191393671Illumina Single End Adapter 1 (95% over 22bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2086010.18895398420066026No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTCGTATG2062510.1868253181689943No Hit
AGTGACGAAAAATAACAATACAGGACCTGTAGGCACCATCAATTCGTATG2017190.18272016308154318No Hit
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATTCGTATGCC1929050.17473630673979687No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1879800.17027516622662459No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT1861810.16864560444323434No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCCTGTAGGCACCATCAATTCGT1856280.16814468856429338No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA1846050.16721803947901923No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA1806510.16363644565382465Illumina Single End Adapter 1 (95% over 22bp)
TATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCGTATGCCGT1801380.16317176238818865No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATTCGTA1770630.1603863802403704No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA1757370.15918526910931124Illumina Single End Adapter 1 (95% over 22bp)
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACCTGTAGGCACCATCA1741820.1577767262670812No Hit
AGTGCCAGGTGGGGAGTTTGACTGGGGCCTGTAGGCACCATCAATTCGTA1709480.15484731948137578No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATTCG1685100.15263894170043893No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1612900.146098954998895No Hit
ACGTGTTAGGACCCGAAAGATGGTGAACTCTGTAGGCACCATCAATTCGT1574680.14263692879760678No Hit
AGTGACGAAAAATAACAATACAGGACTGTAGGCACCATCAATTCGTATGC1568740.14209887449002823No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCTGTAGGCACCATCAATTCGTA1483780.1344030674240563No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC1344530.1217895889172697No Hit
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTCTGTAGGCACCATCAATTC1333040.12074880710008494No Hit
TAGTGACGAAAAATAACAATACAGGACCTGTAGGCACCATCAATTCGTAT1309020.11857303867112254No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT1304210.11813734149613048No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCTGTAGGCACCATCAATT1295070.11730942628211231No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATTC1290450.1168909395984401No Hit
AGAATCCCGCCCAGGCGGAACGATACGCTGTAGGCACCATCAATTCGTAT1246840.11294067893286766No Hit
TAGTGACGAAAAATAACAATACAGGACTGTAGGCACCATCAATTCGTATG1245730.1128401334309464No Hit
TACCTGGTTGATCCTGCCAGTAGCATCTGTAGGCACCATCAATTCGTATG1242890.11258288187648124No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTATG1234350.11180931558242051No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCCTGTAGGCACCATCAATTCGTATGC1204700.1091235731211909No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA1198390.10855200364630528Illumina Single End Adapter 1 (95% over 22bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATTC1186640.10748767063047228No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTCTGTAGGCACCATCAATTCGTATG1184430.10728748544196244No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATTCGTA1171440.1061108313248841No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATTCGTATGCCGT1165790.10559904566195165No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAA1145450.1037566172753948No Hit
AGGACCCGAAAGATGGTGAACTATGCCTGGGCCTGTAGGCACCATCAATT1111200.10065419976115825No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)