FastQCFastQC Report
Wed 28 Jun 2023
SRR970565.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR970565.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194516225
Total Bases9.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA37662301.9362035223539835No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT30812291.5840472947693696No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT24543621.2617775201014723No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT17351540.8920356129675044No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG15735050.8089325196394286No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC15533220.7985565214418489No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA14262470.7332277808702077No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT8951860.4602114810731084No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT6822190.35072601270151116No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT6543590.33640330003319774No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG6496030.3339582597801289No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC5523920.28398248012473No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCG4728890.24311031123496252No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA4562080.23453467699159797No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCCTGTAGGCACCATCA4447460.22864210941786473No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT4439080.22821129702676474No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTC4195970.21571311082147518No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATTC4151100.2134063623741413No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG4074800.20948381041221625No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC3876070.19926718195358767No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATTC3842490.197540847813595No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT3825400.19666225786563563No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT3743030.19242764967292575No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCCTGTAGGCACCATCAATTCG3682580.18931993976337963No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGCACCATCAATT3422000.1759236279647109No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG3084800.15858831313428995No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTCCTGTAGGCACCATCAATTCG2896800.14892330961080497No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATT2831860.14558477062774583No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC2751300.14144321379874608No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2641300.13578815854564316No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTCG2617600.13456975118656553No Hit
GACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTAT2527820.12995419790816934No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC2404820.1236308179433361No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2387170.12272343862317911No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT2361830.12142071953123706No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTA2326800.11961984148108981No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGACTGTAGGCACCATCAAT2304610.11847906260775933No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCTGTAGGCACCATCAATT2264960.11644067223698178No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATTCG2189820.11257775540318038No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATGC1992290.10242281845640384No Hit
AACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT1979640.10177248710229699No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA1951340.10031759561445325No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)