FastQCFastQC Report
Wed 28 Jun 2023
SRR970587.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR970587.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91743213
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA12406041.3522569783990452No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC11932701.3006629711126423No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT8984850.9793476494005067No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATGC7374910.8038643686917745No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT6751060.7358647881669459No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC5905460.6436944823373474No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA5145520.560861106968207No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT5084170.5541739638004612No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC4519480.4926228166872682No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT4442800.48426470522675064No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT4101150.4470248932746665No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT3438800.3748288170373976No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCG2975990.3243825785783195No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT2774860.3024594309771994No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGTAT2567150.2798190641088622No Hit
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATG2422890.264094740174404No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATTC2300830.2507902137676386No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATTC2110330.23002573498270656No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGCACCATCAATT2090100.22782066723562427No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATG1921120.20940186605411343No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATTC1894530.20650355901531375No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATT1796000.19576379998812554No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATTCGTA1733100.18890770699299578No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTCGTATG1675490.18262822340874416No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATT1671480.18219113385531854No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATTCG1614670.17599885018197478No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATTC1549050.16884627748975828No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT1540810.16794811840740742No Hit
GACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTATGC1506080.16416255227512036No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATT1504210.16395872248337323No Hit
CTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATT1481330.1614648050314087No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA1477930.16109420541005035No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTCG1456730.15878340777099229No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1438880.15683775975886086No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1434490.15635925024775402No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCGT1404880.1531317635452772No Hit
CGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTAT1404230.15306091361766455No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATTCGT1312160.14302529387105725No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTCG1263990.13777476923551826No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT1263080.1376755793368606No Hit
AAGATTAGCATGGCCCCTGCGCAAGGATGACACTGTAGGCACCATCAATT1260570.1374019896163872No Hit
TATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCGTATGCCGT1256670.13697689005071143No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGTATGC1252790.13655397048280835No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT1241410.13531355175014417No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCG1228420.13389764319677794No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGGACCATCAATTCGT1198680.13065598650877858No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTCCTGTAGGCACCATCAATTCG1183550.12900681819373386No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATTCGT1159840.12642243083420243No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATT1154360.12582511144448363No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG1148270.12516130212269763No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT1143680.12466099263386382No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC1135020.12371705359828634No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGCACCATCAATTC1134030.12360914370853787No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATTCGTA1121000.12218887515962625No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCCTGTAGGCACCATCAATTCGT1101570.1200710073234518No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCCTCAATTCGT1058090.11533169216561012No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTA1055980.11510170240059064No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCCTGTAGGCACCATCAATTCG1048870.11432671319239712No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGTATG1046800.11410108342292306No Hit
GCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATTCGTATG1034230.11273095482278346No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATTC1034060.11271242484171554No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATTC1014080.11053460706679195No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT979680.10678501089775437No Hit
ACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGC976000.10638389130757823No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTA962820.10494727277537141No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGCTGTAGGCACCATCAATTCGTA946850.10320654455387343No Hit
GATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTCGT932720.1016663761274635No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)