FastQCFastQC Report
Thu 29 Jun 2023
SRR970588.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR970588.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218657879
Total Bases11.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT55256732.5270861609336293No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA29489061.3486392594158476No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT28554881.305915896129222No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA28418801.29969247529379No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC19228680.8793957065686163No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGCACCATCAATT16802550.7684401804702404No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA16125720.7374863450495648No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT13925760.6368743748767453No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATTC13025280.595692232064503No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG12602260.5763460277596492No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCG12384200.5663733708859401No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATTCG10889650.49802230085658156No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC9857410.4508143061243176No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT9358370.427991437710781No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT9109190.4165955529093923No Hit
TAAGATTAGCATGGCCCCTGCGCAAGGATGACACTGTAGGCACCATCAAT9042700.4135547294867888No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGCACCATCAAT8167600.37353330405258345No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT8102770.37056839831506827No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGACTGTAGGCACCATCAAT7441930.34034584228268305No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT6934440.31713652541191983No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCA6636400.3035060995904017No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT6605080.30207372495367524No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGTATG6217120.28433093874472276No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATT5912350.27039272616378024No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT5840140.26709030686243873No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTC5587440.2555334399818266No Hit
AACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT5069960.23186724499417646No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG5036450.23033471389338778No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGCACCATCAATT4978490.22768399761162963No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGTAT4873360.22287603000118736No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCCTGTAGGCACCATCAATTCG4871900.22280925902514584No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGTATGC4846020.22162567487449195No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGCACCATCAATTC4806500.21981828516684734No Hit
AAGATTAGCATGGCCCCTGCGCAAGGATGACACTGTAGGCACCATCAATT4746320.21706604041466987No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTCCTGTAGGCACCATCAATTCG4686600.21433483309330004No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC4683510.21419351643852724No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT4579850.20945277713957886No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA4534510.20737921819867283No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC4530260.20718485063142864No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATT4487350.20522242420544104No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTCG4404170.20141830791288337No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGTAT4258840.19477185178403747No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAA3839650.1756008069574296No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATTCGTA3768700.1723560119230828No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATTCGT3606630.16494397624702103No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATTC3447100.15764810377585342No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAGACTGTAGGCACCATCAA3425200.15664653913523052No Hit
TTGGCAGGGGAGATACCATGATCACGAAGGTGGTCTGTAGGCACCATCAA3255210.14887229378091607No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGCTGTAGGCACCATCAATTC3223500.1474220830615484No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC3099970.141772618218802No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATTC3045530.13928288401626726No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCCTGTAGGCACCATCAATTCGT3045100.13926321859181667No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCCTGTAGGCACCATCAAT2983310.1364373428318126No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC2966840.13568411134180994No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATC2888640.1321077481045172No Hit
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATG2882560.13182968814949494No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAAT2879640.13169614619741188No Hit
TGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACTGTAGGCACCATCAA2855300.13058299170641824No Hit
AAAACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCA2754130.1259561289351023No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCTGTAGGCACCATCAATTCGT2705170.12371701456044948No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGTA2688440.12295189234868596No Hit
AACGCGCCCGATCTCGTCTGATCTCGGAAGCCTGTAGGCACCATCAATTC2613230.11951227241164267No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATT2608140.119279488666402No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTAGACTGTAGGCACCATCAAT2594350.11864882307762621No Hit
TCCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATTC2564430.11728047540422726No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCGT2560790.11711400529957577No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT2545560.11641748340566316No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATTCG2503470.11449255848676736No Hit
AACATCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAA2485980.11369267878062607No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCG2481170.1134727004280509No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT2460170.11251229597813853No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG2438160.1115057006475399No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2396340.10959312378585728Illumina Single End Adapter 1 (95% over 22bp)
CTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATT2382690.10896886089341423No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT2379820.10883760561859288No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAGACTGTAGGCACCATCAATT2379700.10883211759316479No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATTC2360600.10795860687919688No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG2350400.10749212471781089No Hit
TAACGATTAAAGTCCTACGTGATCTGAGTTCCTGTAGGCACCATCAATTC2294730.10494613825463843No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA2286890.10458758726000447No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT2235640.10224374306676597No Hit
AGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTACTGTAGGCACCATCA2231180.10203977145502266No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT2196220.10044092671364474No Hit
CGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAA2191040.10020402694933303No Hit
AGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCTGTAGGCACCATCAAT2190190.1001651534358842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)