FastQCFastQC Report
Fri 29 Sept 2023
SRR9730216.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9730216.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27786018
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCGTTAGGCATTAACGCGGCCGCTCTACAATAGTGATC390820.14065347542782128No Hit
CAAGCAGAGCAGGAATGCCGAGACCGATCTCCGATGTTTA342130.1231302736505821Illumina Paired End PCR Primer 2 (95% over 24bp)
AGCCGAGTGGCATTAACGCGAACTCGGCCTACAATAGTTC323640.11647584767273958No Hit
CAGCAGAAGCAGGAATGCCGAGACCGATCTCCGATGTTTA321780.11580644624933302Illumina Paired End PCR Primer 2 (95% over 24bp)
CTATTGTAGGCATTAACGCGAACTCGGCCTACAATAGTGA305500.10994738432833376No Hit
TCGCGTTAGGCATTAACGCGAACTCGGCCTACAATAGTGA287960.10363485692696232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)