FastQCFastQC Report
Thu 29 Jun 2023
SRR974752.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR974752.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158353479
Total Bases8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAA44568572.814498947636004No Hit
TTCTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAA36505922.3053437304020328No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGCTGTAGGCACCATCA15638330.9875583472340385No Hit
TCTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAAT12369670.7811429264525347No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGCTGTAGGCACCATC11443440.7226516317964824No Hit
TCTCTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCA8721930.5507886568125225No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCCTGTAGGCACCATCAATT8620320.5443719995567637No Hit
TGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAATTG8078620.5101637204952093No Hit
TTGCCAAGGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT6881520.43456702331118346No Hit
TTTGCCAAGGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA6622690.4182219450953774No Hit
CTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAATT6612620.4175860260070447No Hit
CTGTAGGCACCATCAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC5836880.36859815375448746RNA PCR Primer, Index 1 (100% over 33bp)
TTGCCAAGGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATT5212220.329150962322716No Hit
TACTCGGATAACCGTAGTAATTCTAGAGCTAATACTGTAGGCACCATCAA5187410.32758421430071644No Hit
TTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATT5007420.31621787103269133No Hit
TGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTCTGTAGGCACCATCAAT4747980.29983427140239843No Hit
CTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATT4708790.2973594283962653No Hit
TTTTGAAATTTCATCGTGATGGGGATAGATCATCTGTAGGCACCATCAAT4608020.29099581702275074No Hit
ACCTCCCGGGAAGTCCTCGTGTTGCATCCCTCTGTAGGCACCATCAATTG3344160.21118323519750395No Hit
CTCTGATGATTCATGATAACTCGACGGATCGCACTGTAGGCACCATCAAT3312140.20916117668624126No Hit
ATTAAATCAGTTATAGTTTGTTTGATGGTACTGTAGGCACCATCAATTGG3259220.20581928610485406No Hit
CTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATTGG3171900.2003050403458455No Hit
TTGGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGCTGTAGGCACCAT3122290.19717217580044452No Hit
TACTCGGATAACCGTAGTAATTCTAGAGCTACTGTAGGCACCATCAATTG2938990.1855968064964332No Hit
GTAACCCATGCTATACTCCCAGGAAAAGCTCGACTGTAGGCACCATCAAT2871370.18132661297577177No Hit
TGCTCTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATC2763880.17453863454430324No Hit
GCCAAGGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATTGG2751510.17375747077839698No Hit
AAATCGAGGCAAACTCTGAATACTAGATATGACCTCTGTAGGCACCATCA2745190.17335836366436888No Hit
TTCTGATGATTCATGATAACTCGACGGATCGCACTGTAGGCACCATCAAT2539900.1603943289430351No Hit
TACTCGGATAACCGTAGTAATTCTAGAGCTAATCTGTAGGCACCATCAAT2392650.15109551208533917No Hit
TTAAATCAGTTATAGTTTGTTTGATGGTACTGTAGGCACCATCAATTGGA2369060.14960580689231337No Hit
CCTCTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCA2336920.147576170397873No Hit
TGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGCTGTAGGCACCATCA2318320.1464015830053219No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCCTGTAGGCACCATCAATTGG2302950.1454309696599719No Hit
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCGCTGTAGGCACCATCAAT2294080.14487083040341664No Hit
ATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAATTGGA2281940.14410419110526773No Hit
ATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAATTGGAATT2251880.14220590631924163No Hit
GAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCCTGTAGGCACCATCAA2248070.14196530535334814No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGGCTGTAGGCACCATC2245810.1418225866701672No Hit
GTAACCCATGCTATACTCCCAGGAAAAGCTCGAACTGTAGGCACCATCAA2225320.1405286460425666No Hit
ATCGAGGCAAACTCTGAATACTAGATATGACCTCTGTAGGCACCATCAAT2167970.1369070015822008No Hit
GTCTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAA2140030.1351425944989816No Hit
CTCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATT2098540.13252250681527497No Hit
TAATCGAGGCAAACTCTGAATACTAGATATGACCTCTGTAGGCACCATCA2058790.13001229988764568No Hit
TACTCGGATAACCGTAGTAATTCTAGAGCTAATACCTGTAGGCACCATCA2035530.1285434341483587No Hit
GATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAATTGGAAT2004800.12660283895625685No Hit
TTGCCAAGGATGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATTG1869220.11804098096259696No Hit
CCGACGCGGATTACGGTGGCGGCCTAAGCCCGCTGTAGGCACCATCAATT1834820.11586862578497564No Hit
TTGTGGAGACGTCGCTGCCGTGATCGTGGTCCTGTAGGCACCATCAATTG1827350.11539689633216078No Hit
TTCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATT1764250.11141214017786119No Hit
CTGATGATTCATGATAACTCGACGGATCGCACTGTAGGCACCATCAATTG1758330.11103829300775891No Hit
TCGAGGCAAACTCTGAATACTAGATATGACCTCTGTAGGCACCATCAATT1736300.10964710159604388No Hit
TGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATTG1728330.10914379721332172No Hit
AGTAAGCCCACCCCAAGATGAGTGCTCTCCCTGTAGGCACCATCAATTGG1696080.10710721423430174No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCTCTGTAGGCACCATCAATTG1656550.10461090027583166No Hit
CATTAAATCAGTTATAGTTTGTTTGATGGTACTGTAGGCACCATCAATTG1620880.1023583447762458No Hit
TTGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAATTGGA1616600.10208806337623944No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTGTAGGCACCATCAATTG1613450.10188914131782352No Hit
GCCAAGGATGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATTGGA1594070.10066529703461709No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)