FastQCFastQC Report
Tue 27 Jun 2023
SRR974753.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR974753.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153191513
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAA42070362.7462591873480617No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCCTGTAGGCACCATCAATT32903292.147853321352078No Hit
TTCTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAA28447961.8570193245627125No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGCTGTAGGCACCATCA11363850.7418067605350958No Hit
TCTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAAT10430020.6808484227190836No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGCTGTAGGCACCATC8858470.5782611468822035No Hit
TCTCTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCA8089700.528077557403588No Hit
CTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATT6241440.407427270465042No Hit
TACGCATTTATTAGATAAAAGGTCGACGCGGGCCTGTAGGCACCATCAAT6097680.39804293857976325No Hit
TGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAATTG5825330.38026453854529135No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTGTAGGCACCATCAATTG5379230.35114412637206605No Hit
CTGTAGGCACCATCAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4242630.2769494155984999RNA PCR Primer, Index 1 (100% over 33bp)
TTTTGAAATTTCATCGTGATGGGGATAGATCATCTGTAGGCACCATCAAT4076790.2661237506022935No Hit
TGCTCTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATC3858660.2518847111327897No Hit
CTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAATT3723290.2430480597185563No Hit
CTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATTGG3369170.21993189661884205No Hit
TGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTCTGTAGGCACCATCAAT3326910.2171732581556264No Hit
TTGGCCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATT3047280.1989196359722617No Hit
GCCAAGGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATTGG3030820.1978451639158365No Hit
TTGCCAAGGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT2884270.18827870705866062No Hit
TTGCCAAGGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATT2805630.18314526340633505No Hit
ACCTCCCGGGAAGTCCTCGTGTTGCATCCCTCTGTAGGCACCATCAATTG2632330.17183262626304893No Hit
TTGGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGCTGTAGGCACCAT2567540.16760327969343838No Hit
CTCTGATGATTCATGATAACTCGACGGATCGCACTGTAGGCACCATCAAT2468570.16114273902366902No Hit
TTTGCCAAGGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA2462450.1607432390853141No Hit
GTAACCCATGCTATACTCCCAGGAAAAGCTCGAACTGTAGGCACCATCAA2257320.14735281059597602No Hit
CCTCTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCA2180570.14234274192461302No Hit
ATTAAATCAGTTATAGTTTGTTTGATGGTACTGTAGGCACCATCAATTGG2079670.1357562151631729No Hit
TACTCGGATAACCGTAGTAATTCTAGAGCTAATACTGTAGGCACCATCAA2033290.13272863229701243No Hit
AAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCTGTAGGCACCATCAATT1920880.12539075842928712No Hit
GTCTGATGATTCATGATAACTCGACGGATCGCATCTGTAGGCACCATCAA1887350.12320199487813661No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGGCTGTAGGCACCATC1877900.12258511997332384No Hit
GTAACCCATGCTATACTCCCAGGAAAAGCTCGACTGTAGGCACCATCAAT1874830.12238471722646933No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCCTGTAGGCACCATCAATTGG1813000.11834859284926574No Hit
TTCTGATGATTCATGATAACTCGACGGATCGCACTGTAGGCACCATCAAT1803920.11775587071850384No Hit
TGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGCTGTAGGCACCATCA1708590.1115329411231809No Hit
TCAGCTGAGGCATCCTAACAGACCGGTAGACTTGACTGTAGGCACCATCA1686300.11007789968103521No Hit
TGACGCATTTATTAGATAAAAGGTCGACGCGGGCCTGTAGGCACCATCAA1656020.10810128887492612No Hit
GAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCCTGTAGGCACCATCAA1629530.1063720808084192No Hit
TTGACGCATTTATTAGATAAAAGGTCGACGCGGGCCTGTAGGCACCATCA1625750.10612533084649409No Hit
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCGCTGTAGGCACCATCAAT1592900.10398095617738302No Hit
TTGTGGAGACGTCGCTGCCGTGATCGTGGTCCTGTAGGCACCATCAATTG1586280.10354881735517554No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)