FastQCFastQC Report
Wed 23 Aug 2023
SRR9855603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33248225
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2009680.6044473050816999Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA408040.12272534849604753No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)