FastQCFastQC Report
Wed 27 Sept 2023
SRR9855604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265420654
Total Bases39.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA43389801.6347559749438338Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT43294831.6311778811305317No Hit
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA42279231.5929140917571547Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG37290831.4049709183521188No Hit
TAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT35344061.3316243279243822No Hit
AAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT34262451.290873542945908Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA24030220.9053636044465476Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA22658740.8536916648543861No Hit
TAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA17650450.6649991149520715Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA16519080.6223735700688915No Hit
TAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC15581160.5870364557236002Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT13369890.5037245518956487No Hit
TAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC13248120.49913674012723963Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC11985570.4515688519100703Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC11972750.4510858450375154Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC11695490.4406397853273318Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAG10367310.3905992184014436No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA9931680.37418640374535433No Hit
AAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC8209870.309315415973619Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC7998760.30136162651456655Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG7649570.2882055290241279No Hit
GGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG6949330.2618232566030826Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT6292610.2370806455777929Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG6229110.23468821684087932Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT6029740.22717674412783265Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC5727670.21579594178831313Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC5684590.21417285785152199Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC5478380.20640368100366446Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA5446060.2051859912906401Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATC5295610.19951763060609443No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG5169490.19476592805019613No Hit
TTGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT5020470.18915144410728488Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC4908240.1849230617900595Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4892030.18431233313139225Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4891750.18430178383932397Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA4712060.177531775654505Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGAGCAGTGGCTGGTTGAGATTTAACTGTAGGCACCATCAATAGATCGG4522830.1704023380185025No Hit
AAAAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC4407470.16605602968637098Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTCTGTAGGCACCATCAAT4390520.165417420755809No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA4281570.1613126158599549No Hit
TGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4121360.15527653699474345Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTAGCAGAGCAGCTCCCTCGCTGCGATCCTGTAGGCACCATCAATAGA3992910.1504370492584198No Hit
TGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGAT3888940.14651987105720868No Hit
TTGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3863830.14557382561494253Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT3817210.14381736848557383Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT3816450.14378873469281708Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3789360.14276809068521096Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGA3718460.14009685922934995No Hit
AGAGCAGTGGCTGGTTGAGATTTAACTGTAGGCACCATCAATAGATCGGA3668090.13819911693835252No Hit
AAAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC3642000.13721614897384737Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATCAAT3596390.1354977446480107No Hit
AGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA3579860.13487495965555113Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3502360.13195506631522352Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGACTGTAGGCACCATCAATAGAT3487360.1313899256687085No Hit
TGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC3476720.13098905257011387Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3475100.13092801738029022Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3439880.12960106714227296Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA3359720.12658095552729667Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTAGGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT3341050.12587754380260097Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA3259760.12281485825892056Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT3173340.11955889461413202Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT3155540.1188882610469342Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC3102540.11689143076258111Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGATC3083920.11618990284004048No Hit
TAGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT3074200.11582369170109873Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA3050440.11492850891701893Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3036370.11439840699058787Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3009660.1133920798793601No Hit
TCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC2951420.11119782712915778Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGATCCTTCGATGTCGGCTCTTCCTATCCTGTAGGCACCATCAATAGA2910070.10963992274693136No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT2858040.10767963822438625No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATA2852560.10747317350819278No Hit
TAGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2821270.10629429011956243Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT2794450.10528381864359358Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTGGCTGGTTGAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA2769570.10434643869124066No Hit
TTGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC2744890.10341659394750793Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTCTGTAGGCACCATCAA2674030.10074686953337098No Hit
TGGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2670100.10059880268398405Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)