FastQCFastQC Report
Wed 23 Aug 2023
SRR9855605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41446577
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT7186791.733988792367582No Hit
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA6762261.6315605508266704Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA6584091.5885726823713329Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG6156481.4854013155296275No Hit
TAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT5887451.4204912507008722No Hit
AAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT5316691.2827814465836347Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA3795500.9157571685594205No Hit
AGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA3735350.9012445104935927Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA2748550.6631548849015927No Hit
TAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA2743630.661967814615909Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2432190.5868253004343399Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC2064570.49812798774673234Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1868250.45076098805457443Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1865120.4500057990313651Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1819220.43893130185395046Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAG1740520.419943002771978No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1597360.3854021527519631No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1433860.3459537804533291No Hit
AAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1274080.3074029491024072Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG1266680.3056175181849155No Hit
TAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1241410.2995205128761297Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1088970.2627406359758009Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1014730.24482842093328963Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT988330.2384587754979139Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCAGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT943330.22760142532397792Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC888850.2144567933800661Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC888160.2142903140107324Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATC879890.2122949743232113No Hit
GGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA856360.20661778655448435Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC847820.20455730276591957Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT789370.19045481126221836Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC767290.18512747144354044Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC765730.18475108330417733Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC757610.18279193478390265Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA746440.18009689919628344Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGAGCAGTGGCTGGTTGAGATTTAACTGTAGGCACCATCAATAGATCGG728950.175877009095347No Hit
AAAAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC687640.1659099616356738Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGAT647530.15623244351397222No Hit
TGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC638950.1541623087474751Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG629990.1520004896906203No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGA625580.15093646937357458No Hit
TTGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC599370.14461266608337764Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT598100.14430624753402435Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT594220.14337010267458275Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGCAGTGGCTGGTTGAGATTTAACTGTAGGCACCATCAATAGATCGGA593660.14323498898352932No Hit
TAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC592810.1430299057024661Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC565730.13649619364223972Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA560130.13514505673170552Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC543120.13104097836595768Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGTAGCAGAGCAGCTCCCTCGCTGCGATCCTGTAGGCACCATCAATAGA543080.1310313273880253No Hit
AAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC541760.13071284511625653Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC539130.13007829331720205Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC532330.12843762706869616Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA526190.12695620195607468Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTAGGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT521700.12587287968316418Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA518250.12504048283649577Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGATC511460.12340222933247297No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA507420.12242748056130184No Hit
TTGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT497320.119990608633374Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT493400.11904481279600002Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGATCCTTCGATGTCGGCTCTTCCTATCCTGTAGGCACCATCAATAGA489140.11801698364620075No Hit
TAGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT481490.11617123411663165Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC480360.11589859399004168Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC472580.11402147878219232Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA470180.11344242010624907Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTCTGTAGGCACCATCAAT459630.11089697467658184No Hit
TCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC458630.11065570022827217Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGACTGTAGGCACCATCAATAGAT450480.10868931347454822No Hit
TAGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC442440.10674946691013833Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT438120.10570716129344047Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTGGCTGGTTGAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA437740.10561547700308278No Hit
CAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT429620.10365632848280812No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA429150.10354292949210257No Hit
TTGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC428130.10329682955482669Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT418410.10095164191725652Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGG414660.10004686273609519No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)