FastQCFastQC Report
Wed 23 Aug 2023
SRR9855606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56594254
Total Bases8.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3953370.6985461810310284Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA683110.1207030664279098Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGCTTCTCGGCCTTTTGGCTAAGATCCTGTAGGCACCATCAATAGATC619910.10953585500040339No Hit
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA614410.10856402489199699Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)