FastQCFastQC Report
Tue 22 Aug 2023
SRR9855607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25432546
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1707280.6712973211569144Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA299170.11763273720216606Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGCTTCTCGGCCTTTTGGCTAAGATCCTGTAGGCACCATCAATAGATC296020.1163941667499589No Hit
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA270640.10641482767788958Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)