FastQCFastQC Report
Tue 22 Aug 2023
SRR9855608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27637744
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1876680.6790279264472527Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA332930.1204620753416053Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGCTTCTCGGCCTTTTGGCTAAGATCCTGTAGGCACCATCAATAGATC305730.11062046164115277No Hit
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA302120.10931427688164418Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA296240.10718675156698752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)