FastQCFastQC Report
Wed 23 Aug 2023
SRR9855610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33469827
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT7571882.2623003100673333No Hit
TAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT6528111.9504462930148996No Hit
AAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG6038541.8041742492424593No Hit
TGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA3924341.1725008318686558No Hit
AAAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA2880560.8606438270505551No Hit
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA2187800.6536633726848962Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA2119310.6332001656297775Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAG1959420.5854287803758292No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1865320.5573139054468372No Hit
AAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1732890.5177469247152069Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1549690.46301105769085693No Hit
TAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG1367000.40842756671553754No Hit
AGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1194900.357008119581855Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATC939320.2806468046578191No Hit
TAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA937260.2800313249303619Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGAGCAGTGGCTGGTTGAGATTTAACTGTAGGCACCATCAATAGATCGG913760.2730100756122821No Hit
TAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC851670.254459038584215Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGA760500.22721957899573247No Hit
ACGTAGCAGAGCAGCTCCCTCGCTGCGATCCTGTAGGCACCATCAATAGA730660.2183040862446047No Hit
TAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC730340.21820847774325214Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGAT724030.21632319760720603No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG702790.2099771833299288No Hit
TAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC654760.1956269448300405Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGCAGTGGCTGGTTGAGATTTAACTGTAGGCACCATCAATAGATCGGA653930.19537896027965726No Hit
TGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC606070.18107951379611253Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC589260.17605707970943502Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGATC578220.17275858641277111No Hit
TTTGATCCTTCGATGTCGGCTCTTCCTATCCTGTAGGCACCATCAATAGA577210.17245682208037705No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA551000.16462588826646757No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTCTGTAGGCACCATCAAT533030.15925687336238697No Hit
TCGGTCGTCCGACCTGGGTATAGGGGCGACTGTAGGCACCATCAATAGAT527570.15762555330805864No Hit
TCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATCAAT467340.13963024069410337No Hit
CAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT449780.13438372418238073No Hit
TAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGATC433100.1294001310493777No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA430150.12851874017753365No Hit
TAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC427140.12761942271168597Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGAACTGTAGGCACCATCAATAGA424840.12693223660821432No Hit
AAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC412320.12319155399279477Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGG406990.12159907489214092No Hit
TTTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATA393330.1175177869906528No Hit
TTTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAG372780.11137792854441703No Hit
GAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT369920.11052342756357839No Hit
GGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG361850.1081123006700931Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATA348540.10413558456695937No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTCTGTAGGCACCATCAA342810.10242359483961479No Hit
TAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATC340840.101835005003163No Hit
TTGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT340820.10182902947182845Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG337000.10068770298693208Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)